炎症性肠病不同病程阶段上皮细胞的细胞差异组成

封面


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

论证。由于炎症性肠病的病例数量不断增加，而且缺乏可靠的征兆，因此需要建立一定的形态学鉴别诊断标准。

这项研究的目的是确定 Crohn 病和溃疡性结肠炎恶化期和缓解期肠上皮细胞的差异组成。

材料和方法。研究对象为炎症性肠病患者（Crohn 病，30 人；溃疡性结肠炎，30 人）和肠易激综合征患者（对比组，15 人）的组织材料。研究采用了组织学、免疫组化和统计学方法。

结果。在所有列出的病种中，回肠、升结肠、乙状结肠和直肠粘膜上皮细胞差异子组成的特殊性均已确定。此外，还对炎症性肠病的加重期和缓解期进行了评估。结果发现，褐藻细胞在上皮细胞中所占的比例因肠道科室、病名和病程阶段而异。

梭形细胞分化：与缓解期相比，Crohn 疾病加重期回肠隐窝之间的表面上皮粘液细胞增加了 25.0%（p=0.0002）；与肠易激综合征相比，升结肠表面上皮中的梭形细胞增加了 42.9%（p=0.0001）；与溃疡性结肠炎急性期相比，乙状结肠中的隐窝梭形细胞增加了 23.0%（p=0.0024）。Paneth 细胞的数量差异没有统计学意义。与肠易激综合征相比，Crohn 病急性期乙状结肠内分泌细胞的数量高出 3 倍（p=0.0238）。

非上皮细胞：缓解期与恶化期相比，Crohn 疾病的上皮内淋巴细胞数量更少--乙状结肠为 2.4 倍，直肠为 4.0 倍（p<0.0001）；与肠易激综合征相比--回肠为 4.8 倍，升结肠为 2.7 倍，乙状结肠为 4.0 倍（p<0.05）；与缓解期溃疡性结肠炎相比--直肠为 8.0 倍（p=0.0004）。研究发现，与溃疡性结肠炎相比，Crohn 病急性期乙状结肠肠隐窝上皮细胞（主要是梭形细胞）的增殖活性增加了 4.2 倍（p=0.0016）。

结论。在确定肠上皮细胞分化组成时，已经建立了可用作鉴别诊断标准的形态计量参数。与溃疡性结肠炎恶化期相比，Crohn 疾病恶化期的特点是乙状结肠隐窝上皮的增殖指数增加。与同一阶段的溃疡性结肠炎相比，缓解期 Crohn 疾病的特点是直肠上皮内淋巴细胞数量较少。

全文:

受限制的访问

作者简介

Liudmila I. Bernardelli

North-Western State Medical University named after I.I. Mechnikov

Email: bernardellimila@gmail.com
ORCID iD: 0000-0001-9077-7718
SPIN 代码: 5671-1891

doctor

俄罗斯联邦, 47 Piskarevsky Prospekt, 195067 Saint Petersburg

Fedor A. Indeickin

National Center for Clinical Morphological Diagnostics

Email: f.indeickin@yandex.ru
ORCID iD: 0000-0002-1436-2235
SPIN 代码: 4627-4445

doctor of pathological anatomy

俄罗斯联邦, Saint Petersburg

Lilia G. Matyusheva

North-Western State Medical University named after I.I. Mechnikov

Email: liliali2719@gmail.com
ORCID iD: 0000-0003-1746-8059
SPIN 代码: 3658-3376

student of the faculty of Preventive Medicine

俄罗斯联邦, 47 Piskarevsky Prospekt, 195067 Saint Petersburg

Alexey M. Emelin

Petrovsky National Research Centre of Surgery

Email: eamar40rn@gmail.com
ORCID iD: 0000-0003-4109-0105
SPIN 代码: 5605-1140

doctor of pathological anatomy

俄罗斯联邦, Moscow

Maria I. Skalinskaya

North-Western State Medical University named after I.I. Mechnikov

Email: Mariya.Skalinskaya@szgmu.ru
ORCID iD: 0000-0003-0769-8176
SPIN 代码: 2596-5555

MD, Cand. Sci. (Medicine), Assistant Professor

俄罗斯联邦, 47 Piskarevsky Prospekt, 195067 Saint Petersburg

Anna S. Nekrasova

North-Western State Medical University named after I.I. Mechnikov

Email: Anna.Nekrasova@szgmu.ru
ORCID iD: 0000-0001-5198-9902
SPIN 代码: 7502-5036

MD, Cand. Sci. (Medicine), Assistant Professor

俄罗斯联邦, 47 Piskarevsky Prospekt, 195067 Saint Petersburg

Roman V. Deev

Petrovsky National Research Centre of Surgery

编辑信件的主要联系方式.
Email: romdey@gmail.com
ORCID iD: 0000-0001-8389-3841
SPIN 代码: 2957-1687

MD, Cand. Sci. (Medicine), Assistant Professor

俄罗斯联邦, Moscow

参考

  1. Ocansey DKW, Wang L, Wang J, et al. Mesenchymal stem cell-gut microbiota interaction in the repair of inflammatory bowel disease: an enhanced therapeutic effect. Clin Transl Med. 2019;8(1):31. doi: 10.1186/s40169-019-0251-8
  2. Maev IV, Shelygin YuA, Skalinskaya MI, et al. The pathomorphosis of inflammatory bowel diseases. Annals of the Russian Academy of Medical Sciences. 2020;75(1):27–35. EDN: FWJIAO doi: 10.15690/vramn1219
  3. Tkachev AV, Mkrtchyan LS, Mazovka KE, Bohanova EG. In the labyrinths of pathogenesis: the environment and metamorphosis of IBD. South Russian Journal of Therapeutic Practice. 2021;2(3):30–39. EDN: KZOSKG doi: 10.21886/2712-8156-2021-2-3-30-39
  4. Kononova YA, Likhonosov NP, Babenko AY. Metformin: expanding the scope of application-starting earlier than yesterday, canceling later. Int J Mol Sci. 2022;23(4):2363. doi: 10.3390/ijms23042363
  5. Yu Y, Yang W, Li Y, Cong Y. Enteroendocrine cells: sensing gut microbiota and regulating inflammatory bowel diseases. Inflamm Bowel Dis. 2020;26(1):11–20. doi: 10.1093/ibd/izz217
  6. Ocansey DKW, Zhang L, Wang Y, et al. Exosome-mediated effects and applications in inflammatory bowel disease. Biol Rev Camb Philos Soc. 2020;95(5):1287–1307. doi: 10.1111/brv.12608
  7. Fernández-Tomé S, Ortega Moreno L, Chaparro M, Gisbert JP. Gut microbiota and dietary factors as modulators of the mucus layer in inflammatory bowel disease. Int J Mol Sci. 2021;22(19):10224. doi: 10.3390/ijms221910224
  8. Kovaleva AL, Poluektova EA, Shifrin OS. Intestinal barrier, permeability and nonspecific inflammation in functional gastrointestinal disorders. Russian Journal of Gastroenterology, Hepatology, Coloproctology. 2020;30(4):52–59. EDN: EAPBNM doi: 10.22416/1382-4376-2020-30-4-52-59
  9. Ng QX, Soh AYS, Loke W, et al. The role of inflammation in irritable bowel syndrome (IBS). J Inflamm Res. 2018;11:345–349. doi: 10.2147/JIR.S174982
  10. Kirsch R, Riddell R. Histopathological alterations in irritable bowel syndrome. Mod Pathol. 2006;19(12):1638–1645. Corrected and republished from: Mod Pathol. 2007;20(2):278. doi: 10.1038/modpathol.3800704
  11. Lockhart A, Mucida D, Bilate AM. Intraepithelial Lymphocytes of the Intestine. Annu Rev Immunol. 2024;42(1):289–316. doi: 10.1146/annurev-immunol-090222-100246
  12. Tavakoli P, Vollmer-Conna U, Hadzi-Pavlovic D, Grimm MC. A review of inflammatory bowel disease: a model of microbial, immune and neuropsychological integration. Public Health Rev. 2021;42:1603990. doi: 10.3389/phrs.2021.1603990
  13. Sharapov IY, Kvaratskheliiya AG, Bolgucheva MB, Korotkikh KN. Functional morphology of goblet cells of the small intestine under the influence of various factors. Journal of Anatomy and Histopathology. 2021;10(2):73–79. EDN: NEDOPW doi: 10.18499/2225-7357-2021-10-2-73-79
  14. Churkova ML, Kostyukevich SV. The epithelium mucosal of colon in normal and in functional and inflammatory bowel diseases. Experimental and Clinical Gastroenterology Journal. 2018;(5):128–132. EDN: XZJCPJ
  15. Mavlikeev MO, Arkhipova SS, Chernova ON, et al. A short course in histological technique. Kazan: Kazan University; 2020. 107 р. (In Russ.)
  16. Kononov AV, Mozgovoy SI, Shimanskaya AG. Lifetime pathologic and anatomical diagnosis of diseases of the digestive system (class XI ICD-10). Clinical recommendations RPS3.11(2018). Moscow: Practical medicine; 2019. 191 р. (In Russ.)
  17. Ivashkin VT, Shelygin YuA, Abdulganieva DI, et al. Crohn’s disease. Clinical recommendations (preliminary version). Koloproktologia. 2020;19(2):8–38. EDN: AGKJNF doi: 10.33878/2073-7556-2020-19-2-8-38
  18. General Assembly of the World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. J Am Coll Dent. 2014;81(3):14–18.
  19. Singh V, Johnson K, Yin J, et al. Chronic inflammation in ulcerative colitis causes long-term changes in goblet cell function. Cell Mol Gastroenterol Hepatol. 2022;13(1):219–232. doi: 10.1016/j.jcmgh.2021.08.010
  20. Kang Y, Park H, Choe BH, Kang B. The role and function of mucins and its relationship to inflammatory bowel disease. Front Med (Lausanne). 2022;9:848344. doi: 10.3389/fmed.2022.848344
  21. Zolotova NA, Akhrieva KM., Zayratyants OV. Epithelial barrier of the colon in health and patients with ulcerative colitis. Experimental and Clinical Gastroenterology Journal. 2019;(2):4–13. EDN: SUBULS doi: 10.31146/1682-8658-ecg-162-2-4-13
  22. Rezzani R, Franco C, Franceschetti L, et al. A focus on enterochromaffin cells among the enteroendocrine cells: localization, morphology, and role. Int J Mol Sci. 2022;23(7):3758. doi: 10.3390/ijms23073758
  23. Tao E, Zhu Z, Hu C, et al. Potential roles of enterochromaffin cells in early life stress-induced irritable bowel syndrome. Front Cell Neurosci. 2022;16:837166. doi: 10.3389/fncel.2022.837166
  24. Knyazev OV, Shkurko TV, Fadeyeva NA, et al. Epidemiology of chronic inflammatory bowel disease. Yesterday, today, tomorrow. Experimental and Clinical Gastroenterology Journal. 2017;(3):4–12. EDN: ZRPJFX

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Endocrine cells in the epithelium of the mucous membrane of the rectum, immunohistochemical reaction with antibodies to chromogranin A, staining of nuclei with hematoxylin; ×400: a — irritable bowel syndrome, cytoplasmic reaction; b — Crohn's disease in remission, cytoplasmic reaction; c — Crohn's disease in the acute stage, cytoplasmic reaction; d — ulcerative colitis in remission, cytoplasmic reaction; e — ulcerative colitis in the acute stage, cytoplasmic reaction.

下载 (4MB)
索引源数据
3. Fig. 2. Interepithelial lymphocytes (МЭЛ) (*) in the epithelium of the mucous membrane of the sigmoid colon, immunohistochemical reaction with antibodies to (CD3), staining of nuclei with hematoxylin; ×1000: a — irritable bowel syndrome, cytoplasmic reaction; b — Crohn's disease remission, membrane reaction, c — Crohn's disease in the acute stage, membrane reaction; d — ulcerative colitis in remission, membrane reaction; e — ulcerative colitis in the acute stage, cytoplasmic reaction; f — diagram; * p <0.05.

下载 (2MB)
索引源数据
4. Fig. 3. Proliferation in the epithelium of the mucous membrane of the ileum, immunohistochemical reaction with antibodies to Ki-67, staining of nuclei with hematoxylin; ×400: a — irritable bowel syndrome; b — Crohn's disease in remission; c — Crohn's disease in acute stage; d — ulcerative colitis in remission; e — ulcerative colitis in acute stage; f — diagram; * p <0.05.

下载 (2MB)
索引源数据

版权所有 © Eco-Vector, 2024


Периодический печатный журнал зарегистрирован как СМИ Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор): № 0110212 от 08.02.1993.
Сетевое издание зарегистрировано как СМИ Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор): ЭЛ № ФС 77 - 84733 от 10.02.2023.