INTERRELATION BETWEEN THE PREOPERATIVE MORPHOLOGICAL CHARACTERISTICS OF PERIPHERAL BLOOD AND THE QUALITY OF ENGRAFTMENT OF VASCULARIZED ADIPO-FASCIO-CUTANEOUS FLAPS IN RATS



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Abstract

The aim of this work was to determine the interrelation between the preoperative blood counts and the quality of vascularized adipo-f ascio-cutaneous flap survival. In 10 adult male Wistar rats, a flap with the area of 18 cm2 was formed on the basis of superficial inferior epigastric artery; after a 90 min ischemic period it was replanted. 12 days after operation the quality of flap survival was assessed with computer-assisted planimetry. It was found that the combination of preoperative leukocyte, thrombocyte, neutrophil and eosinophil counts as well as reactive lymphocyte counts close to the maximal reference values increased the probability of epidermolysis.

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About the authors

N. A Shchudlo

G. A. Ilizarov Russian Scientific Center «Restorative Traumatology and Orthopaedics»

Email: nshchudlo@mail.ru
Clinical and Experimental Laboratory of Reconstructive Microsurgery and Hand Surgery

M. M. Shchudlo

G. A. Ilizarov Russian Scientific Center «Restorative Traumatology and Orthopaedics»

Email: m.m.sch@mail.ru
Clinical and Experimental Laboratory of Reconstructive Microsurgery and Hand Surgery

L. I. Sbrodova

G. A. Ilizarov Russian Scientific Center «Restorative Traumatology and Orthopaedics»

Clinical and Experimental Laboratory of Reconstructive Microsurgery and Hand Surgery

References

  1. Абрамов М. Г. Гематологический атлас. М.: Медицина, 1985.
  2. Гайдышев И. П. Анализ и обработка данных: специальный справочник. СПб.: Питер, 2001.
  3. Шихалева Н. Г., Щудло Н. А., Щудло М. М., Борисова И. В. Гистологические изменения сосудов и предпосылки феномена «no-reflow» при повреждениях кисти и предплечья циркулярной пилой // Бюл. Восточно-Сибирск. науч. центра СО РАМН. 2011. № 4, ч. 1. С. 206-209.
  4. Щудло М. М., Ступина Т. А., Щудло Н. А. Количественный анализ метахромазии суставного хряща в телепатологии // Изв. Челябинск. НЦ (УРО РАН). 2004. Вып. 25. С. 17-22.
  5. Узенбаева Л. Б., Виноградова И. А., Голубева А. Г. и др. Возрастные изменения лейкоцитарной формулы и морфометрических параметров больших гранулярных лимфоцитов крови крыс при различных режимах освещения // Успехи геронтол. 2006. Вып. 19. С. 79-84.
  6. Brevard P. B. Beta-Carotene increases monocyte numbers in per ipheral rat blood // Int. J. Vitam. Res. 1994. Vol. 64, № 1. P. 21-25.
  7. Desyatnikova S., Winslow C., Cohen J. I., Wax M. K. Effect of Anemia on the Fasciocutaneous Flap Survival in a Rat Model // The Laryngoscope. 2001. Vol. 111, I. 4. P. 572-575.
  8. Davison S. P., Clemens M. W., Kochuba A. L. Anatomy of Free Flap Failures: Dissection of a Series // Sci. Res. 2013. Vol. 3, № 3. P. 89-95.
  9. Gormus Z. I., Ergene N., Toy H. et al. Preventive role of mag nesium on skeletal muscle ischemia-reperfusion injury-an experimental study // Biol. Trace Elem. Res. 2009. Vol. 127, № 2. P. 183-189.
  10. Hassanpour S. E., Mohammadkhah N., Arasteh E. Is it safe to extract the reverse sural artery flap from the proximal third of the leg? // Arch. Iran Med. 2008 Vol. 11, № 2. P. 179-185.
  11. Krammer C. V., Ibrahim R. M., Hansen T. G., Sorensen J. A. The effect of epinephrine and dobutamine on skin flap viability in rats: a randomized double-blind placebo-controlled study // Plast. Reconstr. Surg. 2015. Vol. 68, № 1. P. 113-119.
  12. Levy R. A., Johari V.P., Pantanowitz L. Non-Neoplastic Disorders of White Blood Cells // Non-Neoplastic Hematopathology and Infections. Hoboken: John Wiley & Sons, Inc., 2012.
  13. Moura T.D., Marques A. A., Bernal S. O. et al. Study of the effect of streptokinase and allopurinol in island skin flaps submitted to prolonged ischemia: experimental study in rats // Rev. Assoc. Med. Bras. 2009. Vol. 55, № 5. P. 601-605.
  14. Pereira C. M., Figueiredo M. E., Carvalho R. et al. Anesthesia and surgical microvascular flaps // Rev. Bras. Anestesiol. 2012. Vol. 62, № 4. P. 563-579.
  15. Rodrigues S. F., Granger D. N. Role of blood cells in ischaemia- reperfusion induced endothelial barrier failure // Cardiovasc. Res. 2010. Vol. 87. P. 291-299.
  16. Sengupta P. The laboratory rat: relating its age with human’s // Int J. Prev. Med. 2013. Vol. 4, № 6. P. 624-630.

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