INTERSTRAIN DIFFERENCES IN THE PARAMETERS OF SPERMATOGENESIS IN INBRED MICE
- Authors: Osadchuk L.V.1,2, Kleshyov M.A.1,2
-
Affiliations:
- RAS SB Institute of Cytology and Genetics
- Novosibirsk State Agrarian University
- Issue: Vol 149, No 2 (2016)
- Pages: 54-57
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/397653
- DOI: https://doi.org/10.17816/morph.397653
- ID: 397653
Cite item
Abstract
The aim of the present work was a comparative study of basic parameters of spermatogenesis in mice of inbred PT (n=99) and CBA/Lac (n=81) strains, starting with the puberal period of development until Day 80. The number of spermatozoa was measured in the caudal portions of both epididymides (epididymal reserve), morphological characteristics and spermatozoa motility were evaluated, and body, testes and epididymal weight was determined. Starting from puberty and thereafter, it was found that CBA/Lac males as compared with RT mice had lower testicular mass and epididymal spermatozoa number. However, spermatozoa functional capacity of CBA/Lac males was higher as compared to that of PT mice, since starting with the puberal period, they were characterized by a lower frequency of abnormal forms, and in the post-puberal period - by higher relative concentrations of motile spermatozoa. Thus, in the males of laboratory mice studied, the interstrain differences in the key parameters of spermatogenesis related to fertility, began to emerge in the puberal period, and their interstrain variability may have a compensatory character.
Full Text
About the authors
L. V. Osadchuk
RAS SB Institute of Cytology and Genetics; Novosibirsk State Agrarian University
Email: losadch@bionet.nsc.ru
Sector of Endocrinological Genetics; Scientific Research Department
M. A. Kleshyov
RAS SB Institute of Cytology and Genetics; Novosibirsk State Agrarian University
Email: max82cll@bionet.nsc.ru
Sector of Endocrinological Genetics; Scientific Research Department
References
- Осадчук Л. В. Тестикулярная функция у мышей инбредных линий BALB/cLAC, PT и CBA/Lac // Росс. физиол. журн. им. И. М. Сеченова. 2010. Т. 96, № 2. С. 183-190.
- Осадчук Л. В., Тупикин А. Е., Морозов И. В. и др. Фенотипическая вариабельность сперматогенеза и поиск ассоциаций с генным полиморфизмом у мышей 13 инбредных линий // Генетика. 2012. Т. 48, № 8. С. 966-975.
- Borg C. L., Wolski K. M., Gibbs G. M., O’Bryan M. K. Phenotyping male infertility in the mouse: how to get the most out of a ‘nonperformer’ // Hum. Reprod. Update. 2010. Vol. 16. P. 205-224.
- Chapin R. E., Sloane R. A., Haseman J. K. The relationships among reproductive endpoints in Swiss mice, using the reproductive assessment by Continuous Breeding database // Fundam. Appl. Toxicol. 1997. Vol. 38. P. 129-142.
- Cooper T. G. Sperm maturation in the epididymis: a new look at an old Problem // Asian J. Androl. 2007. Vol. 9. P. 533-539.
- Gomendio M., Malo A. F., Garde J., Roldan E. R. S. Focus on determinants of male fertility. Sperm traits and male fertility in natural populations // Reproduction. 2007. Vol. 134. P. 19-29.
- Holdcraft R. W., Braun R. E. Hormonal regulation of spermatogenesis // Int. J. Androl. 2004. Vol. 27. P. 335-342.
- Perreault S. D., Cancel A. M. Significance of incorporating measures of sperm production and function into rat toxicology studies // Reproduction. 2001. Vol. 121. P. 207-216.
- Robb G. W., Amann R. P., Killian G. J. Daily sperm production and epididymal sperm reserves of pubertal and adult rats // J. Reprod. Fertil. 1978. Vol. 54. P. 103-107.
- Sharpe R. M., McKinnell C., Kivlin C., Fisher J. S. Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood // Reproduction. 2003. Vol. 125. P. 769-784.
- Tarulli G. A., Stanton P.G., Meachem S. J. Is the adult Sertoli cell terminally differentiated? // Biol. Reprod. 2012. Vol. 87. P. 1-11.