MORPHOMETRIC PROPERTIES OF DORSAL CLARKE’S NUCLEI IN ROSTRAL SEGMENTS OF LUMBAR PORTION OF SPINAL CORD IN CAT



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Abstract

Morphometric properties of Clarke’s nuclei and their distribution in gray matter of the cat’s spinal cord were investigated using the methods of histochemical acetylcholinesterase demonstration, immunolabelling of non-phosphorylated domains of heavy neurofilament chains (SMI-32) and Klüver-Barrera technique for myelinated fiber demonstration. As a result of this research, averaged metric maps for LI-LIV lumbar segments of the spinal cord with Clarke’s nuclei boundaries were constructed based on the data received on 5 cats. The work provides information essential for precise stereotaxic access to Clarke’s nuclei in future fundamental and applied studies.

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About the authors

N. S. Merkulyeva

I. P. Pavlov Institute of Physiology

Email: mer-natalia@yandex.ru

A. A. Veshchitskiy

I. P. Pavlov Institute of Physiology

Email: veschickiyalex@mail.ru

P. Yu. Shkorbatova

I. P. Pavlov Institute of Physiology

Email: polinavet@yandex.ru

B. S. Shenkman

Email: bshenkman@mail.ru

P. E. Musienko

Research Institute of Phthisiopulmonology; Institute of Biomedical Problems

Email: pol-spb@mail.ru

F. N. Makarov

I. P. Pavlov Institute of Physiology

Email: felixmakarov@mail.ru

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