POSTNATAL MORPHOGENESIS OF PURKINJE CELLS IN RAT CEREBELLUM
- Authors: Zimatkin S.M.1, Karniushko O.A.1, Ostrovskaya O.B.1
-
Affiliations:
- Grodno State Medical University
- Issue: Vol 152, No 4 (2017)
- Pages: 12-16
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398133
- DOI: https://doi.org/10.17816/morph.398133
- ID: 398133
Cite item
Abstract
Using electron microscopical and histochemical methods, the development of the organelles was examined quantitatively in the cerebellar Purkinje cells of the outbred rats (n=36) in the postnatal period of ontogenesis (Days 2, 7, 15, 45). The study has demonstrated a decrease of nuclear-cytoplasmic ratio, an increase of the nucleolar area, enlargement and lengthening of mitochondria, an increase of the number of membrane-bound ribosomes and of the length of rough endoplasmic reticulum cisterns, an increase in the number and size of lysosomes and the relative area they occupy in the sections of cell cytoplasm.
Keywords
Full Text
About the authors
S. M. Zimatkin
Grodno State Medical University
Email: smzimatkin@mail.ru
Department of Histology, Cytology and Embryology
O. A. Karniushko
Grodno State Medical University
Email: karnyushko-olga@mail.ru
Department of Histology, Cytology and Embryology
O. B. Ostrovskaya
Grodno State Medical University
Email: Astrowskaja@gmail.com
Department of Histology, Cytology and Embryology
References
- Будко К. П., Гладкович Н. Г., Максимова Е. В. Нейроонтогенез. М.: Наука, 1985.
- Карнюшко О. А., Зиматкин С. М. Нарушения морфогенеза коры мозжечка потомства крыс с экспериментальным холестазом и их коррекция // Весцi НАН Беларусi. Сер. мед. навук. 2015. № 3. С. 95-101.
- Оленев С. Н. Развивающийся мозг. Л.: Наука, 1978.
- Altman J. Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layer // J. Comp. Neurol. 1972. Vol. 145, № 4. P. 399-463.
- Altman J., Winfree A. T. Postnatal development of the cerebellar cortex in the rat. V. Spatial organization of Purkinje cell perikarya // J. Comp. Neurol. 1977. Vol. 171, № 1. P. 1-16.
- Andjus P. R., Zhu L., Cesa R. et al. A change in the pattern of activity affects the developmental regression of the Purkinje cell polyinnervation by climbing fibers in the rat cerebellum // Neuroscience. 2003. Vol. 121, № 3. P. 563-572.
- Asari M. A., Abdullan M. S., Ismail Z. I. Histomorphometric study on the effect of low dose deltamethrin on the developing cerebellar cortex // Turk. J. Med. Sci. 2010. Vol. 40, № 6. P. 943-948.
- Castejon О.J. Correlative microscopy of Purkinje cells // Biocell. 2011. Vol. 35, № 3. P. 1-29.
- Lewandowska E., Stępień T., Wierzba-Bobrowicz T. et al. Alcohol-induced changes in the developing cerebellum. Ultra structural and quantitative analysis of neurons in the cerebellar cortex // Folia Neuropathol. 2012. Vol. 50, № 4. P. 397-406.
- Millonig G. Аdvanvantges of a phosphate buffer or OsO4 solutions in fixation // J. Appl. Physics. 1961. Vol. 32. P. 1637-1643.
- Paxinos G., Watson C. The rat brain in stereotaxic coordinates, 6th ed. London: Academic Press, 2007.
- Reynolds E. S. The use of lead citrate at high pH as an electron opaque stain in electron microscopy // J. Cell Biol. 1963. Vol. 17. P. 208-212.