GLUTAMINE SYNTHETASE IN RAT BRAIN CELLS



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Abstract

Objective - to study, using immunohistochemical method, the cells in the brain producing glutamine synthetase (GS). Material and methods. The enzyme was demonstrated in the frontal sections of rat brain (n=10) using monoclonal mouse antibody. The preparations were analyzed using light and confocal laser microscopy. Results. The study showed ubiquitous expression of GS in the brain mainly by two morphologically and topographically different cell types. According to their structure and localization, as well as the immunopositive reaction to glial fibrillary acidic protein, the dominant type of cells was identified as astrocytes. Cells of another type had morphological characteristics and localization corresponding to those of typical oligodendrocytes. Conclusions. Thus, the data obtained suggest that GS is not a selective marker of any cell population of the rat brain.

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About the authors

Ye. G. Sukhorukova

RAS Institute of experimental Medicine

Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

V. V Gusel’nikova

RAS Institute of experimental Medicine

Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

D. E. Korzhevskiy

RAS Institute of experimental Medicine

Email: dek2@yandex.ru
Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

References

  1. Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г., Петрова Е. С., Кирик О. В., Григорьев И. П. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии // Морфология. 2013. Т. 143, № 2. С. 81-85.
  2. Сухорукова Е. Г., Коржевский Д. Э., Алексеева О. С. Глиальный фибриллярный кислый белок - компонент промежуточных филаментов астроцитов мозга позвоночных // Журн. эволюц. биохим. 2015. Т. 51, № 1. С. 3-10.
  3. Anlauf E., Derouiche A. Glutamine synthetase as an astrocytic marker: its cell type and vesicle localization // Front Endocrinol. (Lausanne). 2013. Vol. 4, № 144. P. 1-5.
  4. Bak L. K., Schousboe A., Vaagepetersen H. S. The glutamate/ GABA-glutamine cycle: aspects of transport, neurotransmitter homeostasis and ammonia transfer // J. Neurochem. 2006. Vol. 98, № 3. P. 641-653.
  5. Bernstein H.-G., Bannier J., Meyer-Lotz G. et al. Distribution of immunoreactive glutamine synthetase in the adult human and mouse brain. Qualitative and quantitative observations with special emphasis on extra-astroglial protein localization // J. Chemical Neuroanat. 2014. Vol. 61-62. P. 33-50.
  6. Cammer W. Glutamine synthetase in the central nervous system is not confined to astrocytes // J. Neuroimmunol. 1990. Vol. 26. P. 173-178.
  7. D’Amelio F., Eng L. F., Gibbs M. A. Glutamine synthetase immunoreactivity is present in oligodendroglia of various regions of the central nervous system // Glia. 1990. Vol. 3, № 5. P. 335-341.
  8. Fernandes S. P., Dringen R., Lawen A., Robinson S. R. Neurones express glutamine synthetase when deprived of glutamine or interaction with astrocytes // J. Neurochem. 2010. Vol. 114, № 5. P. 1527-1536.
  9. Hertz L., Zielke H. R. Astrocytic control of glutamatergic activity: astrocytes as stars of the show // Trends Neurosci. 2004. Vol. 27, № 12. P. 735-743.
  10. Korzhevskii D. E., Sukhorukova E. G., Kirik O. V., Grigorev I. P. Immu nohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde // Europ J. Histochem. 2015. Vol. 59, № 3. P. 5-9.
  11. Norenberg M. D., Martinez-Hernandez A. Fine structural localization of glutamine synthetase in astrocytes of rat brain // Brain Res. 1979. Vol. 161, № 2. P. 303-310.
  12. Rose C. F., Verkhratsky A., Parpura V. Astrocyte glutamine synthetase: pivotal in health and disease // Biochem Soc Trans. 2013. Vol. 41, № 6. P. 1518-1524.

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