Email this article CYTOARCHITECTURE OF THE SENSORIMOTOR CORTEX AND CA1 FIELD OF THE HIPPOCAMPUS IN THE BRAIN OF ALBINO RATS WITH DIFFERENT SEIZURE ACTIVITY IN POST-TRAUMATIC PERIOD
- Authors: Sokolov Y.Y.1, Semchenko V.V.1
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Affiliations:
- Omsk P. A. Stolypin State Agrarian University
- Issue: Vol 153, No 1 (2018)
- Pages: 61-64
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398195
- DOI: https://doi.org/10.17816/morph.398195
- ID: 398195
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Abstract
Objective - to study the cytoarchitecture of the sensorimotor cortex (SMC) and CA1 field of the hippocampus of albino rat brain after severe traumatic brain injury. Material and methods . The polytrauma was modeled according to Noble-Collip method under the anesthesia. The main group (n=25) included animals with convulsive paroxysms (CP) and the comparison group (n=25) - rats free of them. The material was collected on days 1, 3, 7, 14 and 30 after the injury. The brain sections were stained with Nissl method, total numerical density of pyramidal neurons (TNDN) and the proportion of dark neurons (DN) were determined. Verification of statistical hypotheses was performed using Statistica 8.0. Results . One day after trauma, the number of DN was significantly increased in SMC and CA1 field of the hippocampus. In the late recovery period, in the comparison group 65.0% of DN in SMC restored to normochromic cells, while in CA1 field of the hippocampus this was found in 18% neurons. The emergence of CP prevented the restoration of DN: in SMC 54.5% of the neurons, and in CA1 field of the hippocampus - only 2.7% of the cells were restored. As a result, in the main group TNDN in SMC decreased by 32.4%, while in CA1 field of the hippocampus it was reduced by 55.6% and in the comparison group - by 24.7% and 43.3%, respectively. Conclusions. CP are a factor contributing to the pathological changes of neurons in the post-traumatic period.
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About the authors
Ye. Yu. Sokolov
Omsk P. A. Stolypin State Agrarian University
Email: sokolovnew22@yandex.ru
Department of Anatomy, Histology, Physiology and Pathological Anatomy, Institute of Veterinary Medicine and Biotechnologies
V. V. Semchenko
Omsk P. A. Stolypin State Agrarian UniversityDepartment of Anatomy, Histology, Physiology and Pathological Anatomy, Institute of Veterinary Medicine and Biotechnologies
References
- Боровиков В. Statistica. Искусство анализа данных на компьютере. СПб.: Питер. 2003. 2-е изд. 688 с.
- Семченко В. В., Степанов С. С., Боголепов Н. Н. Синаптическая пластичность головного мозга (фундаментальные и прикладные аспекты). 2-е изд. М.: Медиа-Сфера, 2014. 408 с.
- Kroemer G., Galluzzi L., Vandenabeele P. et al. Classification of cell death: recommendations of the Nomenclature Committee on Cell Death 2009 // Cell Death Differ. 2009. Vol. 16, № 1. P. 3-11.
- Maurer L. L., Philbert M. A. The mechanisms of neurotoxicity and the selective vulnerability of nervous system sites // Handb. Clin. Neurol. 2015. Vol. 131. P. 61-70.
- Noble R. L., Collip J. B. A quantitative method of production of experimental trauma without hemorrhage in unanesthetized animals // Q. J. Exp. Physiol. 1942. Vol. 31. P. 187-202.
- Zille M., Farr T. D., Przesdzing I. Visualizing cell death in experimental focal cerebral ischemia: promises, problems, and perspectives // J. Cereb. Blood Flow Metab. 2012. Vol. 32. P. 213-231.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).