METHODOLOGICAL PECULIARITIES OF THE MORPHOMETRIC CHARACTERIZATION OF HUMAN NEOCORTEX SYNOPTOARCHITECTONICS BASED ON IMMUNOFLUORESCENT DEMONSTRATION OF NEUROMODULIN

Objective - to explore the possibilities of morphometric characteristics of synoptoarchitectonics using GAP-43. Material and methods. Used immunofluorescent detection of neuromodulin (GAP-43), confocal microscopy and automated computer image analysis (ImageJ 1.46. program) were used to study the layer V (field 4) of human cerebral cortex (n=4). Results. Application of imunofluorescent demonstration of GAP-43 has allowed to detect the distribution of axodendritic and axosomatic synapses, the total area of axonal terminals, numerical density of medium and large terminals. Conclusion. A necessary condition for obtaining accurate data is high resulting magnification of digital images (600- 900 pixels/inch). The findings obtained should be taken into account when examining synoptoarchitectonics of the neocortex using the immunohistochemical methods for studying the structure of synapses.

neocortex, synapses, neuromodulin, immunofluorescence, automated computer analysis