Email this article DEMONSTRATION OF WEIBEL-PALADE BODIES USING VON WILLEBRAND FACTOR IMMUNOCYTOCHEMISTRY AND CONFOCAL LASER MICROSCOPY
- Authors: Sukhorukova Y.G.1, Kirik O.V.1, Korzhevskiy D.E.1
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Affiliations:
- RAS Institute of Experimental Medicine
- Issue: Vol 153, No 1 (2018)
- Pages: 71-75
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398199
- DOI: https://doi.org/10.17816/morph.398199
- ID: 398199
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Abstract
Objective - to explore the possibility of application of immunocytochemical reaction to von Willebrand factor to analyze the localization of Weibel-Palade bodies in the venous endothelium using confocal laser microscopy. Material and methods. The study was performed on paraffin sections of human great saphenous vein (n=5). Results. A comparative study of preparations for light and confocal laser microscopy suggests that the confocal laser microscopy due to the possibility to achieve high resolution allows identification of the localization of the product in more detail, as well as to carry out a quantitative analysis. Conclusions. The presented set of reagents for immunofluorescent reaction provides high quality preparations, in which individual Weibel-Palade bodies are readily identifiable Application of the proposed set of fluorochromes for confocal laser microscopy allows easy separation of channels and the study of structures independently of each other.
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About the authors
Ye. G. Sukhorukova
RAS Institute of Experimental Medicine
Email: len48@inbox.ru
Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology
O. V. Kirik
RAS Institute of Experimental Medicine
Email: iemmorphol@yandex.ru
Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology
D. E. Korzhevskiy
RAS Institute of Experimental Medicine
Email: dek2@yandex.ru
Laboratory of Functional Morphology of Central and Peripheral Nervous System, Department of General and Special Morphology
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).