STRUCTURAL AND BIOMECHANICAL CHARACTERISTICS OF BLOOD NEUTROPHILS IN RATS EXPOSED TO TITANIUM DIOXIDE NANOPARTICLES (ATOMIC FORCE MICROSCOPIC STUDY)



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Abstract

Objective - to study the structural and biomechanical characteristics of blood neutrophils after exposure to titanium dioxide (TiO2) nanoparticles. Materials and methods. Blood neutrophils obtained from 20 healthy donors of both sexes aged 20 to 22 years were studied in vitro before and after exposure to TiO2 nanoparticles. Using atomic force microscopy, structural and biomechanical characteristics of neutrophils (diameter, cell height, roughness, stiffness) were examined. Results. The structural changes of neutrophils after exposure to TiO2 nanoparticles were observed which consisted of decline of cell diameter and an increase of neutrophil height, rigidity and degree of roughness of their surface. Conclusion. The changes in the structural and biomechanical properties of neutrophils observed indicate the possible cytotoxic effect of TiO2 nanoparticles.

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About the authors

L. A. Sharafutdinova

Bashkir State University

Email: sharafla@yandex.ru
Department of Human Physiology and General Biology 32 Zaki Validi Str., Ufa 450076

Yu. S. Zamula

Bashkir State University

Laboratory of Experimental Research of the Center for Micro-nanoscale Dynamics of Dispersed Systems 32 Zaki Validi Str., Ufa 450076

Z. R. Khismatullina

Bashkir State University

Department of Human Physiology and General Biology 32 Zaki Validi Str., Ufa 450076

M. R. Daminov

Bashkir State University

Department of Human Physiology and General Biology 32 Zaki Validi Str., Ufa 450076

V. V. Valiullin

Kazan’ State Medical University

Department of Histology 49 Butlerova Str., Kazan’ 420012

References

  1. Гайнитдинова В. В., Шарафутдинова Л. А., Камалтдинов И. М., Авдеев С. Н. АСМ-исследование структурнофункциональных особенностей нейтрофилов у больных хронической обструктивной болезнью легких в фазе обострения // Клиническая лабораторная диагностика. 2015. Т. 60, № 5. С. 17-22.
  2. Bogdan J., Pławińska-Czarnak J., Zarzyńska J. Nanoparticles of titanium and zinc oxides as novel agents in tumor treatment: a review// Nanoscale Res. Lett. 2017. Vol. 12, № 1. P. 225.
  3. Dulinska I., Targosz M., Strojny W. et al. Stiffness of normal and pathological erythrocyte studied by means of atomic force microscopy// J. Biochem. Biophys. Methods. 2006. Vol. 9, № 1-3. P. 1-11.
  4. Fröhlich E. Cellular targets and mechanisms in the cytotoxic action of non-biodegradable engineered nanoparticles // Curr. Drug. Metab. 2013. Vol. 14, № 9. P. 976-988.
  5. Horie M., Nishio K., Fujita K. et al. Protein adsorption of ultra-fine metal oxide and its influence on cytotoxicity toward cultured cells // Chem. Res. Toxicol. 2009. Vol. 22, № 3. P. 543-553.
  6. Katsnelson B., Privalova L., Degtyareva T. et al. Experimental estimates of the toxicity of iron oxide Fe3O4 (magnetite) nanoparticles // Cent. Eur. J. Occup. Environ. Med. 2010. Vol. 16. P. 47-63.
  7. Lekka M., Fornal M., Pyka-Fosciak G. et al. Erythrocyte stiffness probed using atomic force microscope // Biorheology. 2005. Vol. 9, № 4. P. 307-317.
  8. Ma-Hock L., Burkhardt S., Strauss V. et al. Development of a short-term inhalation test in the rat using nano-titanium dioxide as a model substance // Inhal. Toxicol. 2009. Vol. 21, № 2. P. 102-118.
  9. Nogueira D. R., Rolim B., Vinardell M. Mechanisms underlying cytotoxicity induced by engineered nanomaterials: a review of in vitro studies // Nanomaterials (Basel). 2014. Vol. 4, № 2. Р. 454-484.
  10. Ogneva I., Buravkov S., Shubenkov A., Buravkova L. Mechanical characteristics of mesenchymal stem cells under impact of silica-based nanoparticles// Nanoscale Res. Lett. 2014. Vol. 9. P. 28410.
  11. Park E., Yi J., Chung K. et al. Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells // Toxicol. Lett. 2008. Vol. 180. P. 222-229.
  12. Privalova L., Katsnelson B., Loginova N. et al. Some characteristics of free cell population in the airways of rats after intratracheal instillation of copper-containing nano-scale particles // Int. J. Mol. Sci. 2014. Vol. 5, № 11. P. 21 538-21 553.
  13. Roca-Cusachs P., Almendros I., Sunyer R. et al. Rheology of passive and adhesion-activated neutrophils probed by atomic force microscopy // Biophys J. 2006. Vol. 91. № 9. P. 3508- 3518.
  14. Wang Q., Huang J., Li H., Zhao A. et al. Recent advances on smart TiO2 nanotube platforms for sustainable drug delivery applications // J. Nanomedicine. 2016. Vol. 12. P. 151-165.
  15. Zheng Y., Wen J., Nguyen J. et al. Decreased deformability of lymphocytes in chronic lymphocytic leukemia // Sci. Rep. 2015. Vol. 5. P. 7613.

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