CHANGES OF H3 HISTONE ACETYLATION IN THE HIPPOCAMPUS OF RATS RESULTING FROM SEVERE HYPOBARIC HYPOXIA AND THE ROLE OF HYPOXIC POSTCONDITIONING



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Abstract

Objective: to study the effects of severe hypobaric hypoxia (SHH) and SHH followed by moderate hypobaric hypoxia in the regime of.neuroprotective postconditioning (PostC) on H3 histone acetylation level in rat hippocampus neurons. Materials and methods: Quantitative immunohistochemical method was used for estimation of the overall histone acetylation and histone H3 acetylation by Lys9 (acH3K9) which influences the conformational properties of chromatin and gene expression in the neurons of the pyramidal layer in CA1 subfield of hippocampus in response to different modes of hypoxic exposures. Results: SHH did not cause the substantial differences in acH3K9 level at least during 4 days after exposure. PostC by mild hypobaric hypoxia significantly upregulated acH3K9 level already at Day 1 after the first episode, possibly triggering the adaptive responses caused by PostC. Conclusions: processes of histone, in particular, H3 histone acetylation, play an important role in neuroprotective effect of hypoxic PostC.

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About the authors

O. V. Vetrovoy

Russian Academy of Sciences I. P. Pavlov Institute of Physiology; St. Petersburg State University

Email: vov210292@yandex.ru
Laboratory of Regulation of Brain Neuron Function; Department of Biochemistry 6 Makarova Nab., St. Petersburg 199034; 7-9 Universitetskaya Nab., St. Petersburg 199034

T. S. Glushchenko

Russian Academy of Sciences I. P. Pavlov Institute of Physiology

Laboratory of Regulation of Brain Neuron Function 6 Makarova Nab., St. Petersburg 199034

K. V. Sariyeva

Russian Academy of Sciences I. P. Pavlov Institute of Physiology

Laboratory of Regulation of Brain Neuron Function 6 Makarova Nab., St. Petersburg 199034

Ye. I. Tyul’kova

Russian Academy of Sciences I. P. Pavlov Institute of Physiology

Laboratory of Regulation of Brain Neuron Function 6 Makarova Nab., St. Petersburg 199034

Ye. A. Rybnikova

Russian Academy of Sciences I. P. Pavlov Institute of Physiology

Laboratory of Regulation of Brain Neuron Function 6 Makarova Nab., St. Petersburg 199034

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