METHODICAL APPROACHES TO EXPERIMENTAL STUDY OF HISTOPHYSIOLOGY OF MUCOCILIARY TRANSPORT SYSTEM IN THE UTERINE TUBES



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective - to develop the methods for experimental study of the mucociliary system of the uterine tubes based on the use of modern digital technologies. Materials and methods. Using the complex of histological and specialized “Azimuth” (Russia) hardware-software system, the structural and functional organization of the uterine tubes of small laboratory animals was examined. Results and Conclusions. The methodical approaches are proposed which allow to carry out the complex morpho-functional analysis including the intravital study of the mucus flow velocity at the level of the organ and in the parietal layer, the parameters of motor activity of the ciliary apparatus, the linear sizes of microscopic objects in combination with the histological examination of the uterine tubes.

Full Text

Restricted Access

About the authors

A. V. Pavlov

Yaroslavl’ State Medical University

Email: pavlov@ysmu.ru
Department of Histology, Cytology and Embryology 5 Revolutsionnaya St., Yaroslavl’ 150000

T. V. Korablyova

Yaroslavl’ State Medical University

Department of Histology, Cytology and Embryology 5 Revolutsionnaya St., Yaroslavl’ 150000

L. I. Yesev

Yaroslavl’ State Medical University

Department of Histology, Cytology and Embryology 5 Revolutsionnaya St., Yaroslavl’ 150000

O. A. Fokanova

Yaroslavl’ State Medical University

Department of Histology, Cytology and Embryology 5 Revolutsionnaya St., Yaroslavl’ 150000

Yu. A. Lukashevich

P. G. Demidov Yaroslavl’ State University

Email: lukashevich.yuriy@gmail.com
Department of Infocommunications and Radiophysics, Faculty of Physics 14 Sovetskaya St., Yaroslavl’ 150003

References

  1. Алексеев Д. С., Попечителев Е. П. Методы исследования двигательной активности мукоцилиарного аппарата // Известия ЮФУ. Технические науки. Тематический выпуск. 2012. С. 189-194.
  2. Кобылянский В. И. Мукоцилиарная система. Фундаментальные и прикладные аспекты. М.: БИНОМ. 2008. 416 с.
  3. Козлов В. С., Крамной А. И., Аверин А. А., Лукашевич Ю. А., Алексеев Д. С. Исследование двигательной активности цилиарного аппарата мерцательного эпителия полости носа in vitro // Российская ринология. 2005. № 3. С. 30-33.
  4. Павлов А. В., Есев Л. И. Методические подходы к комплексному изучению функциональной морфологии эпителиальной выстилки трахеи в эксперименте // Морфология, 2012. Т. 142, вып. 6. С.73-76.
  5. Bylander A., Nutu M., Wellander R., Goksör M., Billig H., Larsson D. G. Rapid effects of progesterone on ciliary beat frequency in the mouse fallopian tube // Reprod. Biol. Endocrinol. 2010. Vol. 8. P. 48. doi: 10.1186/1477-7827-8-48.
  6. Ezzati M., Djahanbakhch O., Arian S., Carr B. R. Tubal transport of gametes and embryos: a review of physiology and pathophysiology.// J. Assist.Reprod. Genet. 2014. Vol.31, № 10. P. 1337-1347. doi: 10.1007/s10815-014-0309-x.
  7. Kölle S., Dubielzig S., Reese S., Wehrend A., König P., Kummer W. Ciliary transport, gamete interaction, and effects of the early embryo in the oviduct: ex vivo analyses using a new digital videomicroscopic system in the cow // Biol. Reprod. 2009. Vol. 81, № 2. P. 267-274. doi: 10.1095/biolreprod.108.073874.
  8. Lyons R.A., Saridogan E., Djahanbakhch O. The reproductive significance of human Fallopian tube cilia // Hum. Reprod. 2006. Vol. 12, № 4. P. 363-372.
  9. Noreikat K., Wolff M., Kummer W., Kölle S. Ciliary activity in the oviduct of cycling, pregnant, and muscarinic receptor knockout mice // Biol. Reprod. 2012. Vol. 86, № 4. P. 1-10. doi: 10.1095/ biolreprod.111.096339.]
  10. Raidt J., Werner C., Menchen T., Dougherty G. W., Olbrich H., Loges N. T., Schmitz R., Pennekamp P., Omran H. Ciliary function and motor protein composition of human fallopian tubes // Hum. Reprod. 2015. Vol. 30, № 12. P. 2871-2880. doi: 10.1093/humrep/dev227.
  11. Shi D., Komatsu K., Uemura T., Fujimori T. Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct // Genes Cells. 2011. Vol. 16, № 3. P. 282-290. doi: 10.1111/j.13652443.2011.01484.x.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2019 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies