Email this article MORPHO-FUNCTIONAL CHARACTERISTIC OF THE DUODENAL CRYPT EPITHELIUM IN THE OFFSPRING OF FEMALE RATS WITH EXPERIMENTAL LIVER INJURY
- Authors: Serysheva O.Y.1, Briukhin G.V.1
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Affiliations:
- Chelyabinsk State Medical Academy
- Issue: Vol 144, No 4 (2013)
- Pages: 036-040
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398688
- DOI: https://doi.org/10.17816/morph.398688
- ID: 398688
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Abstract
The quantitative characteristics and qualitative composition of the crypt epithelium of the duodenal mucosa was studied in 90 Wistar rats aged 1–60 days which were born to females with experimental D-galactosamine-induced liver injury. Histological sections were stained with hematoxylin–eosin, toluidine blue to demonstrate the goblet cells and impregnated with silver nitrate to identify the endocrine cells. The linear dimensions of the intestinal crypts (height and width of the crypts in their longitudinal sections) were determined. The total number of epithelial cells, microvillous enterocytes, endocrine goblet cells, and intraepithelial lymphocytes were counted per longitudinal section through the crypt. Linear dimensions of microvillous enterocytes (height, width), and the volume of their nuclei were calculated. In experimental animals, as compared to the control ones (the offspring of intact mothers; n=90), the linear dimensions of the crypts were unchanged. These animals had the increased number of the microvillous enterocytes, goblet and endocrine cells along with the decreased dimensions of the the microvillous enterocytes and their nuclear volume. At the same time, these animals demonstrated an increase in the nuclear hyperchromism of the epithelial cells and the increment of the intraepithelial lymphocyte numbers.
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About the authors
O. Yu. Serysheva
Chelyabinsk State Medical Academy
Email: 22kiti22@mail.ru
G. V. Briukhin
Chelyabinsk State Medical Academy
Email: kanc@chelsma.ru
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).