Email this article CALBINDIN-CONTAINING NEURONS OF THE VENTRAL HORN OF MURINE SPINAL CORD GRAY MATTER
- Authors: Porseva V.V.1, Shilkin V.V.1, Strelkov A.A.1, Masliukov P.M.1
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Affiliations:
- Yaroslavl’ State Medical Academy
- Issue: Vol 146, No 4 (2014)
- Pages: 21-25
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398762
- DOI: https://doi.org/10.17816/morph.398762
- ID: 398762
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Abstract
The study was performed in 4 C57black/6 mice to examine the neurons located in T II, L IV, L V and L VI segments of the spinal cord (SC) ventral horn, containing 28 kD calbindin (CAB) and 200 kD neurofilament (NF) proteins. To demonstrate immunoreactive neurons, the cells were labeled with antibodies against CAB and double labeled with antibodies against CAB and NF. The total cell population was demonstrated using NeuroTrace Red Fluorescent Nissl Stain. Results have shown that CABimmunopositive neurons were identified in ventromedial area of the ventral horn at all SC levels and were represented by Renshaw cells. САВ-positive interneurons located in the medial area of the ventral horn were present only in SC lumbar segments. CAB-positive motorneurons that were identified in the medial area of the ventral horn, were present in one SC segment (LIV) and were also found to contain a NF protein.
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About the authors
V. V. Porseva
Yaroslavl’ State Medical Academy
Email: vvporseva@mail.ru
V. V. Shilkin
Yaroslavl’ State Medical Academy
Email: shilkin39@mail.ru
A. A. Strelkov
Yaroslavl’ State Medical Academy
Email: strelkov-yar@mail.ru
P. M. Masliukov
Yaroslavl’ State Medical Academy
Email: mpm@yma.ac.ru
References
- Маслюков П. М., Коробкин А. А., Коновалов В. В. и др. Возрастное развитие кальбиндин-иммунопозитивных нейронов симпатических узлов крысы. Морфология, 2012, т. 141, вып. 1, с. 77-80.
- Порсева В. В., Шилкин В. В., Корзина М. Б. и др. Особенности возрастных изменений НФ200+-нейронов чувствительных узлов различных сегментарных уровней при химической деафферентации. Морфология. 2012, т. 142, вып. 4, с. 37-42.
- Экклс Д. Антидромный тормозной путь. В кн.: Физиология нервных клеток. М.: Изд-во иностр. лит-ры, 1959, с. 182-191.
- Alvarez F. J., Dewey D. E., McMillin P. and Fyffe R. E. W. Distribution of cholinergic contacts on Renshaw cells in the rat spinal cord: a light microscopic study. J. Physiol. 1999, v. 515, Pt. 3, p. 787-797.
- Alvarez F. J. and Fyffe R. E. W. The continuing case for the Renshaw cell. J. Physiol., 2007, v. 584, p. 31-45.
- Anelli R., Heckman C. J. The calcium binding proteins calbindin, parvalbumin, and calretinin have specific patterns of expression in the gray matter of cat spinal cord. J. Neurocytol., 2005, v. 34, № 6, p. 369-385.
- Arvidsson U., Ulfhake B., Cullheim S. et al. Distribution of calbindin D28k-like immunoreactivity (LI) in the monkey ventral horn: do Renshaw cells contain calbindin D28k-LI? J. Neurosci., 1992, v. 12, № 3, p. 718-728.
- Blanchard S. R., Al-Marsoummi S. and Carr Р. А. Renshaw cell loss in a transgenic mouse model of amyotrophic lateral sclerosis. FASEB J., 2009, v. 23, p. 831.
- Capano C. P., Pernas-Alonso R. and Porzio U. Neurofilament homeostasis and motoneurone degeneration. BioEssays., 2001, v. 23, p. 24-33.
- Carr P.A., Alvarez F.J., Leman E. A. and Fyffe R. E. Calbindin D28k expression in immunohistochemically identified Renshaw cells. NeuroReport., 1998, v. 9, № 11, p. 2657-2661.
- Fahandejsaadi A., Leung E., Rahaii R. et al. Calbindin-D28K, parvalbumin and calretinin in primate lower motor neurons. NeuroReport., 2004, v. 15, № 3, p. 443-448.
- Geiman E. J., Knox M. C., Alvarez F. J. Postnatal maturation of gephyrin/glycine receptor clusters on developing Renshaw cells. J. Comp. Neurol., 2000, v. 426, p. 130-142.
- Kim J. S., Kim J. M., Son J. A. et al. Decreased calbindin-immunoreactive Renshaw cells (RCs) in the lumbar spinal cord of the ataxic pogo mice. Korean J. Anat., 2008, v. 41, № 4, p. 255-263.
- Liu Q., Xie F., Siedlak S. L. et al. Neurofilament proteins in neurodegenerative diseases. Cell Mol. Life Sci., 2004, v. 61, p. 3057-3075.
- Meier J., Couillard-Despres S., Jacomy H. et al. Extra neurofilament NF-L subunits rescue motor neuron disease caused by overexpression of the human NF-H gene in mice. J. Neuropathol. Exp. Neurol., 1999, v. 58, p. 1099-1110.
- Megias M., Alvarez-Otero R. and Pombal M. Calbindin and calretinin immunoreactivities identify different types of neurons in the adult lamprey spinal cord. J. Comp. Neurol., 2003, v. 455, № 1, p. 72-85.
- Morona R., Lopez J. M. and Gonzalez A. Calbindin-D28k and calretinin immunoreactivity in the spinal cord of the lizard Gekko gecko: Colocalization with choline acetyltransferase and nitric oxide synthase. Brain Res. Bull., 2006, v. 69, № 5, p. 519-534.
- Morona R., Lopez J. M., Dominguez L. and Gonzalez A. Immunohistochemical and hodological characterization of calbindin-D28k-containing neurons in the spinal cord of the turtle, Pseudemys scripta elegans. Microsc. Res. Tech., 2007, v. 70, № 2, p. 101-118.
- Renshaw B. Central effects of centripetal impulses in axons of spinal ventral roots. J. Neurophysiol., 1946, v. 9, p. 191-204.
- Rexed B. The cytoarchitectonic organization of the spinal cord of the cat. J. Сomp. Neurol., 1952, v. 96, p. 415-495.
- Schmidt H. Three functional facets of calbindin D-28k. Front. Mol. Neurosci., 2012, v. 5, p. 25.
- Schwaller B. The use of transgenic mouse models to reveal the functions of Ca2+ buffer proteins in excitable cells. Biochim. Biophys. Acta, 2012, v. 1820, p. 1294-1303.
- Steiner T. J. and Turner L. M. Cytoarchitecture of the rat spinal cord. J. Physiol., 1972, v. 222, p. 123-125.
- Zhang J. H., Morita Y., Hironaka T. et al. Ontological study of calbindin-D28k-like and parvalbumin-like immunoreactivities in rat spinal cord and dorsal root ganglia. J. Comp. Neurol., 1990, v. 302, p. 715-728.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).