PECULIARITIES OF REGENERATIVE PROCESSES AFTER THE PLASTY OF OSTEOCHONDRAL DEFECTS WITH COMBINED CELL-TISSUE GRAFTS ON THE BASIS OF AUTOLOGOUS AND ALLOGENEIC CELL СULTURES FROM COSTAL CARTILAGE TISSUE

From a position of in vivo regenerative medicine, the efficacy of plasty of osteochondral defects of the articular surface was evaluated in rabbits. The combined cell-tissue grafts were used with the demineralized spongy bone as a biomatrix and autologous or allogeneic cells from the rib cartilage tissue. Morpho-functional characteristics of the cell cultures obtained were studied using morphological, histochemical, immunohistochemical and flow cytometric methods. Cells had features typical to cartilage cell line. While the macroscopic appearance of the plasty area was similar, some differences of histogenesis were observed at the early stages after the placement of transplants containing autologous as well as allogeneic cells, into the osteochondral defect area. In both cases, the complete recovery of subchondral bone and hyaline cartilage took place.

articular cartilage, osteochondral defects, cell-tissue graft, autologous and allogeneic cells