THE DISTRIBUTION OF CHOLINERGIC AND NITROXIDERGIC NEURONS IN THE SPINAL CORD OF NEWBORN AND ADULT RATS



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Abstract

The aim of this study was to examine the distribution of cholinergic and nitroxidergic neurons in the spinal cord (SC) of adult and newborn rats. Using immunohistochemical demonstration of choline acetyltransferase (ChAT) and nitric oxide synthase (NOS), cervical portions of SC were studied in newborn (n=5) and adult (n=5) Wistar rats. It was found that ChAT-positive neurons were localized in the anterior horns of the SC, while individual cells were located in of SC posterior horns, in the central gray matter and at the boundary of VI-VII Rexed laminae. Nitroxidergic neurons were located in the superficial layers of SC posterior horns of grey matter, in the central gray matter and in the area of VI-VII Rexed laminae. It is found that SC of newborn and adult rats contained cholinergic neurons expressing NOS. Detection of cells containing both enzymes already at postnatal Day 1, suggests that they were formed in rat SC during prenatal ontogenesis

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About the authors

Ye. A. Kolos

RAS North-Western Branch Institute of Experimental Medicine

Laboratory of the Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

D. E. Korzhevskiy

RAS North-Western Branch Institute of Experimental Medicine

Email: iemmorphol@yandex.ru
Laboratory of the Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

References

  1. Амахин Д. В., Веселкин Н. П. Взаимодействие эффектов нейромедиаторов глицина и ГАМК в центральной нервной системе // Цитология. 2012. Т. 54, № 6. С. 469-477.
  2. Колос Е. А., Коржевский Д. Э. Выявление нейрональных и глиальных антигенов после декальцинации в растворе муравьиной кислоты и фиксации в цинк-этанол-формальдегиде // Морфология. 2013. Т. 26, вып. 2. С. 236-241.
  3. Коржевский Д. Э., Григорьев И. П., Кирик О. В. и др. Метод иммуноцитохимического определения холинергических нейронов центральной нервной системы лабораторных животных // Морфология. 2013. Т. 143, вып. 6. С. 69-72.
  4. Коржевский Д. Э., Кирик О. В., Гилерович Е. Г. Постнатальный нейроногенез: идентификация клеток и терминология // Морфология. 2013. Т. 144, вып. 4. С. 88-92.
  5. Коржевский Д. Э., Отеллин В. А., Григорьев И. П. и др. Иммуноцитохимическое выявления нейрональной NO-синтазы в клетках головного мозга крысы // Морфология. 2007, Т. 132, вып. 4. С. 77-80.
  6. Коцюба А. Е., Черток В. М. Гистохимическая и иммуногистохимическая локализация холинацетилтрансфераз в ядрах продолговатого мозга крыс // Цитология. 2013. Т. 55, № 11. С. 821-827.
  7. Пущина Е. В., Обухов Д. К. NADPH-диафораза, нейрональная NO-синтаза и тирозингидроксилаза в ядрах промежуточного мозга горчака Rhodeus sericeus (cyprynidae: teleostei) // Цитология. 2010. Т. 52, № 9. С. 739-748.
  8. Alfaro-Cervello C., Soriano-Navarro M., Mirzadeh Z et al. Biciliated ependymal cell proliferation contributes to spinal cord growth // J. Comp. Neurol. 2012. Vol. 520, № 15. P. 3528-3552.
  9. Bolekova A., Kluchova D., Spakovska T. et al. Postnatal development of nitrergic and cholinergic structures in rat spinal cord // Arch. Ital. Biol. 2011. Vol. 149, № 3. P. 293-302.
  10. Dale H. H., Feldberg W., Vogt M. Release of acetylcholine at voluntary motor nerve endings // J. Physiol. 1936. Vol. 89, № 4. P. 353-380.
  11. D’Mello R., Dickenson A. H. Spinal cord mechanisms of pain // Br. J. Anaesth. 2008. Vol. 101, № 1. P. 8-16.
  12. Gibbs S. M. Regulation of neuronal proliferation and differentiation by nitric oxide // Mol. Neurobiol. 2003. Vol. 27, № 2. P. 107-120.
  13. Kluchov D., Schmidtov K., Rybárová S. et al. Partial colocalization of NADPH-diaphorase and acetilcholinesterase positivity in spinal cord neurons // Physiol. Res. 2000. Vol. 49. P. 151-155.
  14. Matarredona E. R., Murillo-Carretero M., Moreno-López B., Estrada C. Nitric oxide synthesis inhibition increases proliferation of neural precursors isolated from the postnatal mouse subventricular zone // Brain Res. 2004. Vol. 995, № 2. P. 274-284.
  15. Matsumoto M., Xie W., Inoue M., Ueda H. Evidence for the tonic inhibition of spinal pain by nicotinic cholinergic transmission through primary afferents // Mol. Pain. 2007. Vol. 3. P. 41-52.
  16. Meller S. T., Gebhart G. F. Nitric oxide (NO) and nociceptive processing in the spinal cord // Pain. 1993. Vol. 52, № 2. P. 127-136.
  17. Mesnage B., Gaillard S., Godin A. G. Morphological and functional characterization of cholinergic interneurons in the dorsal horn of the mouse spinal cord // J. Comp. Neurol. 2011. Vol. 19, № 16. P. 3139-3158.
  18. Moreno-Lуpez B., Noval J. A., Gonzalez-Bonet L. G., Estrada C. Morphological bases for a role of nitric oxide in adult neurogenesis // Brain Res. 2000. Vol. 869, № 1-2. P. 244-250.
  19. Oda Y. Choline acetyltransferase: the structure, distribution and pathologic changes in the central nervous system // Pathol. Int. 1999. Vol. 49. P. 921-937.
  20. Pawlowski S. A., Gaillard S., Ghorayeb I. et al. A novel popu lation of cholinergic neurons in the macaque spinal dorsal horn of potential clinical relevance for pain therapy // J. Neurosci. 2013. Vol. 33, № 9. P. 3727-3737.
  21. Saito S., Kidd G. J., Trapp T. Rat spinal cord neurons contain nitric oxide synthase // Neuroscience. 1994. Vol. 59, № 2. P. 447-456.
  22. Scheiner C., Arceneaux R., Guido W. et al. Nitric oxide synthase distribution in the cat superior colliculus and co-localization with choline acetyltransferase // J. Chem. Neuroanat. 2000. Vol. 18, № 4. P. 147-159.
  23. Watson C., Paxinos G., Kayalioglu G., Heise С. Atlas of the rat spinal cord. In: The Spinal Cord: a Christopher and Dana Reeve Foundation Text and Atlas. London: Acad. Press, 2009. P. 238-306.
  24. Wetts R., Vaughn J. E. Choline acetyltransferase and NADPH diaphorase are coexpressed in rat spinal cord neurons // Neuroscience. 1994. Vol. 63, № 4. P. 1117-1124.
  25. Zagoraiou L., Akay T., Martin J. F. et al. A cluster of cholinergic premotor interneurons modulates mouse locomotor activity // Neuron. 2009. Vol. 64, № 5. P. 645-662.

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