PECULIARITIES OF THE REPARATIVE REGENERATION DURING THE HEALING OF ASEPTIC WOUND USING DIFFERENT METHODS OF THE SURGICAL FIELD PREPARATION OF IN THE EXPERIMENT

The peculiarities of the reparative regeneration after aseptic wound infliction were studied on albino rats (n=24), to detect the effect of the method of prevention of wound infection. Immunohistochemically it was found that unlike the healing of the wounds after treatment of the surgical field by Grossich-Filonchikov method, the application of the exogenous nitric monoxide (NO) for this purpose during the experiment resulted in a statistically significant increase of the number of endothelial cells (2-fold by Day 7). By the same date, the total length of the microvascular bed vessels was also duplicated. Cryofracture identification of caveoli on endotheliocyte replicas indicated the increase in plasmalemmal vesicle density in the endothelium by 6.9%. Additionally, under the condition of NO treatment, the proliferative activity of the cells in the basal layer of the epidermis of the skin was significantly increased, d reaching a maximum by Day 3 days of wound healing and remaining at a high level until the end of the study. These results indicate the positive effect of exogenous NO on the course of the wound process.

aseptic wound, wound process, NO-treatment, endothelium