ANGIOGENESIS IN THE TISSUES AFTER THE INJECTION OF STROMAL STEM CELLS OF BONE MARROW ORIGIN CLOSE TO THROMBOSED VEIN IN AN EXPERIMENT



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Abstract

The effects of the injection of autologous multipotent stromal stem cells of bone marrow origin (MSSCBM) (mesenchymal stem cells) with green fluorescent protein gene, additionally marked with DAPI nuclear stain, close to a thrombosed hindlimb vein, were studied by fluorescent microscopy in adult male Wag rats (n=214). The control groups consisted of intact rats (n=12), animals with venous thrombosis without the injection of MSSCBM (n=71) and rats that received paravasal injection of MSSCBM, but without preliminary modeling of venous thrombosis (n=72). It was found that MSSCBM participated in the development of granulation tissue at the site of surgical intervention performed during the modeling of thrombosis. The rapid development of granulation tissue at the site of surgical trauma may contribute to faster wound clearance from detritus, nonviable tissue and antigenic substances, early onset of tissue repair processes and rapid healing. Restoration of blood flow in the tissue region of a thrombosed vein began later than after the intravenous injection of MSSCBM.

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About the authors

I. V. Maiborodin

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Email: imai@mail.ru
Center of New Medical Technologies

V. V. Morozov

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

Ya. V. Novikova

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

V. A. Matveyeva

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

L. V. Artemiyeva

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

A. L. Matveyev

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

R. V. Maslov

A. I. Yevdokimov Moscow Sate Medico-Stomatologic University

Department of Parodontology, Stomatologic Faculty

N. V. Onopriyenko

Novosibirsk State Medical University

Department of Obstetrics and Gynecology

G. A. Chastikin

RAS Siberian Branch Institute of Chemical Biology and Fundamental Medicine

Center of New Medical Technologies

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