VISUALIZATION OF THREE-DIMENSIONAL RELIEF STRUCTURES OF SEROUS MEMBRANES WITH A LIGHT MICROSCOPE

Methods for three-dimensional visualization of histological film preparations were developed using traditional flat-field microscopes. Preliminarily, the structures examined acquired the light reflecting ability by the reduction of silver nitrate impregnating them. Microscopy of preparations is carried out in reflected incident tenebrous illumination. The methods of processing of preparations for the study of cell surface relief, limiting membrane of the mesothelium and subsurface collagen fibers. Preparations are stored and examined in the wet state

histological preparations, impregnation with silver salts, light microscopy, reflected light microscopy