Email this article DEMONSTRATION OF NITRIC OXIDE SYN-THASE IN HUMAN INTRACARDIAC GANGLIA IN NORMAL AND ISCHEMIC MYOCARDIUM
- Authors: SHUKLIN AV1, SHVALYOV VN1
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Affiliations:
- Russian Cardiologic Scientific Complex, Moscow
- Issue: Vol 129, No 3 (2006)
- Pages: 34-36
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398985
- DOI: https://doi.org/10.17816/morph.398985
- ID: 398985
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Abstract
Nitric oxide synthase (NOS) expression in human intracardiac ganglia was studied using two techniques - histochemical demonstration of NADPH-diaphorase and immunohistochemical staining for NOS1. To detect the influence of coronary heart disease on NOS expression, hearts were studied in patients that died from heart failure (n=8) and in persons that died in accidents (n=3, control). It was found that human intracardiac neurons normally expressed mainly NOS1, and the proportion of these cells amounted to about 40%. A portion of neurons with low and moderate density of staining for NADPH-diaphorase was increased in ischemic myocardium, probably, due NOS2 induction.
About the authors
A V SHUKLIN
Russian Cardiologic Scientific Complex, MoscowRussian Cardiologic Scientific Complex, Moscow
V N SHVALYOV
Russian Cardiologic Scientific Complex, MoscowRussian Cardiologic Scientific Complex, Moscow
References
- Швалев В.Н., Сосунов А.А. и Гуски Г. Морфологические основы иннервации сердца. М., Наука, 1992.
- Armour J.A., Smith F.M., Losier A.M. et al. Modulation of intrinsic cardiac neuronal activity by nitric oxide donors induces cardiodynamic changes. Am. J. Physiol., 1995, v. 268, №2, Pt. 2, p. R403-R413.
- Balligand J.L., Kelly R.A., Marsden P.A. et al. Control of cardiac muscle cell function by an endogenous nitric oxide signaling system. Proc. Natl. Acad. Sci. USA, 1993, v. 90, № 1, p. 347-351.
- Choate J.K., Danson E.J., Morris J.F. and Paterson D.J. Peripheral vagal control of heart rate is impaired in neuronal NOS knockout mice. Am. J. Physiol. Heart. Circ. Physiol., 2001, v. 281, № 6, p. H2310-H2317.
- Danson E.J. and Paterson D.J. Enhanced neuronal nitric oxide synthase expression is central to cardiac vagal phenotype in exercise-trained mice. J. Physiol., 2003, v. 546, Pt. 1, p. 225-232.
- Danson E.J., Zhang Y.H., Sears C.E. et al. Disruption of inhibitory G-proteins mediates a reduction in atrial beta-adrenergic signaling by enhancing eNOS expression: Augmented eNOS signaling in atrial myocytes. Cardiovasc. Res., 2005. v. 67, № 4, p. 613-623.
- Herring N., Danson E.J. and Paterson D.J. Cholinergic control of heart rate by nitric oxide is site specific. News Physiol. Sci., 2002, v. 17, № 10, p. 202-206.
- Klimaschewski L., Kummer W., Mayer B. et al. Nitric oxide synthase in cardiac nerve fibers and neurons of rat and guinea pig heart. Circ. Res., 1992, v. 71, № 6, p. 1533-1537.
- Mohan R.M., Choate J.K., Golding S. et al. Peripheral presynaptic pathway reduces the heart rate response to sympathetic activation following exercise training: role of NO. Cardiovasc. Res., 2000, v. 47, № 1, p. 90-98.
- Richardson R.J., Grkovic I. and Anderson C.R. Immunohistochemical analysis of intracardiac ganglia of the rat heart. Cell Tiss. Res., 2003, v. 314, № 3, p. 337-350.
- Sosunov A.A., Hassall C.J., Loesch A. et al. Nitric oxide synthase-containing neurones and nerve fibres within cardiac ganglia of rat and guinea-pig: an electron-microscopic immunocytochemical study. Cell Tiss. Res., 1996, v. 284, № 1, p. 19-28.
- Tanaka K., Hassall C.J. and Burnstock G. Distribution of intracardiac neurones and nerve terminals that contain a marker for nitric oxide, NADPH-diaphorase, in the guinea-pig heart. Cell Tiss. Res., 1993, v. 273, № 2, p. 293-300.
- Ungureanu-Longrois D., Balligand J.L., Simmons W.W. et al. Induction of nitric oxide synthase activity by cytokines in ventricular myocytes is necessary but not sufficient to decrease contractile responsiveness to beta-adrenergic agonists. Circ. Res., 1995, v. 77, № 3, p. 494-502.
- Vincent S.R. and Kimura H. Histochemical mapping of nitric oxide synthase in the rat brain. Neuroscience, 1992, v. 46, № 4, p. 755-784.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).