CELL PROLIFERATION CHANGES IN BRAIN SUBVENTRICULAR ZONE IN ADULT RATS AFTER в-AMYLOID PEPTIDE (25-35) ADMINISTRATION



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Abstract

The effect of intracerebroventricular administration of the (25-35) fragment of amyloid-в peptide [Ав(25-35)] on cell proliferation in the subventricular zone of the hippocampal dentate gyrus was studied in adult rats. The animals were treated with either Ав(25-35) or Ав(35-25) at a dose of 15 nmol, control rats were injected with a vehicle (sterile water). 1-5 days after peptide administration, the animals were intraperitone-ally injected with a thymidine analogue 5-bromo-2'deoxyuridine (BrdU). Immunohistochemical detection of BrdU incorporation into DNA was performed on the frontal sections of the brains 6 and 12 days after treatment with peptides. 6 days after Ав peptide treatment, the total number of BrdU-positive cells in the subven-tricular zone was not significantly different in all groups studied. The total number of labelled cells was significantly decreased 12 days after surgery. However, in the Ав(25-35)-treated rats the number of BrdU-positive cells was higher as compared to that one in rats injected with control peptide or vehicle. Thus, intra-cerebroventricular administration of Ав(25-35) significantly increased cell proliferation in the subventricular zone.

About the authors

M Yu Stepanichev

Yu V Moiseeva

N A Lazareva

M V Onufriev

N V Gulyaeva

M Yu Stepanichev

RAS Institute of Higher Nervous Activity and Neurophysiology

; RAS Institute of Higher Nervous Activity and Neurophysiology

Yu V Moiseyeva

RAS Institute of Higher Nervous Activity and Neurophysiology

; RAS Institute of Higher Nervous Activity and Neurophysiology

N A Lazareva

RAS Institute of Higher Nervous Activity and Neurophysiology

; RAS Institute of Higher Nervous Activity and Neurophysiology

M V Onufriyev

RAS Institute of Higher Nervous Activity and Neurophysiology

; RAS Institute of Higher Nervous Activity and Neurophysiology

N V Gulyaeva

RAS Institute of Higher Nervous Activity and Neurophysiology

; RAS Institute of Higher Nervous Activity and Neurophysiology

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