Email this article MORPHOLOGICAL CHARACTERISTIC OF ERYTHROCYTES IN EXPERIMENTAL HYPERVITAMINOSIS A
- Authors: Minashkina TA1, Minashkina TA1
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- Issue: Vol 139, No 2 (2011)
- Pages: 41-44
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/399490
- DOI: https://doi.org/10.17816/morph.399490
- ID: 399490
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Abstract
This investigation was aimed at the analysis of the shape and morpho-densitometric parameters of the erythrocytes in rats with experimental hypervitaminosis A. Male Wistar rats received 0.64 mg/g (1167 IU/g) of retinol palmitate (RP) in oil solution orally for 11 consecutive days. Rats fed oil alone and intact animals were used as control groups. At days 5 and 6 of the experiment, the first manifestations of hypervitaminosis A were observed (body mass loss, localized erythema and hemorrhages). In contrast to control groups, in rats with hypervitaminosis A, the area of erythrocyte cytoplasm decreased gradually in response to RP administration. Discocyte/spherocyte/stomatocyte ratio also changed dynamically: the proportion of discocytes progressively decreased, while the amount of spherocytes and stomatocytes increased. These results show that excess of the vitamin A alters the erythrocyte membrane structure. Integral optical density of erythrocyte cytoplasm in RP-treated rats as well as in oil-fed rats was lower than in intact animals. This may be an indirect evidence of the fall in erythrocyte hemoglobin content. The changes observed in erythrocytes of RP-treated rats may serve as an additional criterion for evaluation of hypervitaminosis A severity.
References
- Жукоцкий А.В. Телевизионная компьютерная морфоденситометрия нормальных и патологических структур клеток и тканей: Автореф. дис. … д-ра мед. наук. М., 1992.
- Коколина В.Ф., Картелищев А.В., Альбанова В.И и др. Технологии системной ретинолотерапии в педиатрической практике (методические рекомендации № 10). М., изд. ЗАО «Ретиноиды», 2007.
- Alarcyn-Corredor O.M. La hipervitaminosis A: una enfermedad multisistemica. Rev. Fac. Farmacia, 2006, v. 48, № 2, p. 13-20.
- Hathcock J.N., Hattan D.G., Jenkins M.Y. et al. Evaluation of vitamin A toxicity. Am. J. Clin. Nutr., 1990, v. 52, № 2, p. 183-202.
- Karar P.K., Manavalan R. and Rajagopal G. Erythrocyte, lipid peroxidation and antioxidant enzymes in hypervitaminotic A rats and their modification by dietary protein. Indian J. Exp. Biol., 2002, v. 40, № 11, p. 1250-1253.
- Kartha V.N. and Krishnamurthy S. Effect of hypervitaminosis A on hemolysis and lipid peroxidation in the rat. J. Lipid Res., 1978, v. 19, № 3, p. 332-334.
- Mohandas N. and Gallagher P.G. Red cell membrane: past, present, and future. Blood, 2008, v. 112, № 10, p. 3939-3948.
- Murphy M.J., Jr. Effects of vitamin A on the erythrocyte membrane surface. Blood, 1973, v. 41, № 6, p. 893-899.
- Park Y.K., Best C.A., Badizadegan K. et al. Measurement of red blood cell mechanics during morphological changes. Proc. Natl. Acad. Sci. USA, 2010, v. 107, № 15, p. 6731-6736.
- Penniston K.L. and Tanumihardjo Sh.A. The acute and chronic toxic effects of vitamin A. Am. J. Clin. Nutr., 2006, v. 83, № 2, p. 191-201.
- Perrotta S., Nobili B., Rossi F. et al. Infant hypervitaminosis A causes severe anemia and thrombocytopenia: evidence of a retinol-dependent bone marrow cell growth inhibition. Blood, 2002, v. 99, № 6, p. 2017-2022.
- Schindler R., Gorny C. and Feldheim W. Serum lipoproteins protect isolated erythrocytes against retinol-induced haemolysis. Int. J. Vitam. Nutr. Res., 1985, v. 55, № 3, p. 253-262.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).