Email this article OPTIMIZATION OF THE METHOD FOR IMMUNOCYTOCHEMICAL DEMONSTRATION OF NESTIN IN PARAFFIN SECTIONS OF RAT BRAIN
- Authors: Korzhevskiy DE1, Gilyarov AV1, Korzhevskiy DE2, Gilyarov AV2
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Affiliations:
- RAMS Institute of Experimental Medicine
- Issue: Vol 130, No 6 (2006)
- Pages: 78-80
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/402497
- DOI: https://doi.org/10.17816/morph.402497
- ID: 402497
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Abstract
The aim of this study was to develop an optimal and easy protocol for immunocytochemical demonstration of nestin (an intermediate filament protein which is believed to be a neuronal stem cell marker) in paraffin sections of rat brain with target retrieval procedure being omitted for better preservation of the neural tissue structure. The influence of fixation, character of blocking of nonspecific antibody binding, temperature and time of incubation on the results of immunocytochemical reaction was studied. On the basis of the results obtained, we propose a processing protocol allowing distinct demonstration of nestin in rat brain cells together with the optimal preservation of the nervous tissue structure.
Keywords
About the authors
D E Korzhevskiy
A V Gilyarov
D E Korzhevskiy
RAMS Institute of Experimental Medicine; RAMS Institute of Experimental Medicine
A V Gilyarov
RAMS Institute of Experimental Medicine; RAMS Institute of Experimental Medicine
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Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).