AGE-RELATED CHANGES IN THE VASCULAR PLEXUS OF THE RAT BRAIN ARE ACCOMPANIED BY THE ACCUMULATION OF LIPOFUSCIN IN THE CELLS OF THE INTEGUMENTARY EPITHELIUMAUTHORS



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Abstract

  • BACKGROUND: The choroid plexus of the brain is the main component of the blood-cerebrospinal fluid barrier, consists of stromal and integumentary epithelium, the cells of which belong to a population of slowly renewing tissues and can accumulate lipofuscin with age, similar to other brain cells.
  • AIM: The purpose of this study was to test the hypothesis that the epithelium of the choroid plexus of the rat brain is characterized by the accumulation of lipofuscin during aging.
  • METHODS: The study was performed on male Wistar rats of different ages: 4-5 months (n=3), 18 months (n=3), 28 months (n=3). The presence of lipofuscin in the tissues was assessed by confocal microscopy using its autofluorescence property.
  • RESULTS: In the course of the study, it was shown that with aging, lipofuscin accumulates in the epithelium of the choroid plexus of the brain in rats.
  • CONCLUSION: Further study of the conditions and experimental effects under which the accumulation of lipofuscin may be delayed will allow us to develop a methodology for testing new medical technologies and drugs that slow down the aging of brain structures.

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Fig.1 Fragment of the vascular plexus in the lateral ventricle of the rat brain. Registration of autofluorescence of lipofuscin when excited by lasers with a wavelength of 561 nm (a), 488 nm (b) and 405 nm (c). Separate (a-c) and combined (d) representation of channels. Confocal laser microscopy.

The scale segment is 5 microns.

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About the authors

Olga Kirik

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’

Author for correspondence.
Email: olga_kirik@mail.ru
ORCID iD: 0000-0001-6113-3948
Scopus Author ID: 27171304100
ResearcherId: A-8710-2012

кандидат биологических наук, старший научный сотрудник лаборатории функциональной морфологии центральной и периферической нервной системы

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street

Olga Sergeevna Alekseeva

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’; Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Email: osa72@inbox.ru
ORCID iD: 0000-0001-5688-347X
Scopus Author ID: 7005782741
ResearcherId: B-4180-2012

кандидат биологических наук, научный сотрудник лаборатории функциональной морфологии центральной и периферической нервной системы 

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street; Thorez 44, St. Petersburg, Russia, 194223

Elena A. Fedorova

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’

Email: el-fedorova2014@ya.ru
ORCID iD: 0000-0002-0190-885X
Scopus Author ID: 36901775900
ResearcherId: B-1671-2012

кандидат биологических наук, научный сотрудник лаборатории функциональной морфологии центральной и периферической нервной системы

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street

Murodali Saidalievich Fayzov

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’

Email: fayzov-1994@mail.ru

аспирант лаборатории функциональной морфологии центральной и периферической нервной системы

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street

Anastasiya Alekseevna Beketova

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’

Email: beketova.anastasiya@yandex.ru
ORCID iD: 0009-0002-8659-733X

лаборант-исследователь лаборатории функциональной морфологии центральной и периферической нервной системы

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street

Dmitriy E. Korzhevskii

Federal State Budgetary Scientific Institution ‘Institute of Experimental Medicine’

Email: DEK2@yandex.ru
ORCID iD: 0000-0002-2456-8165
Scopus Author ID: 12770589000
ResearcherId: C-2206-2012

доктор медицинских наук, профессор РАН, заведующий лабораторией функциональной морфологии центральной и периферической нервной системы

Russian Federation, 197022, Saint-Petersburg 12, Acad. Pavlov Street

References

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