GLUTAMINE SYNTHETASE IN RAT BRAIN CELLS



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详细

Objective - to study, using immunohistochemical method, the cells in the brain producing glutamine synthetase (GS). Material and methods. The enzyme was demonstrated in the frontal sections of rat brain (n=10) using monoclonal mouse antibody. The preparations were analyzed using light and confocal laser microscopy. Results. The study showed ubiquitous expression of GS in the brain mainly by two morphologically and topographically different cell types. According to their structure and localization, as well as the immunopositive reaction to glial fibrillary acidic protein, the dominant type of cells was identified as astrocytes. Cells of another type had morphological characteristics and localization corresponding to those of typical oligodendrocytes. Conclusions. Thus, the data obtained suggest that GS is not a selective marker of any cell population of the rat brain.

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作者简介

Ye. Sukhorukova

RAS Institute of experimental Medicine

Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

V. Gusel’nikova

RAS Institute of experimental Medicine

Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

D. Korzhevskiy

RAS Institute of experimental Medicine

Email: dek2@yandex.ru
Laboratory of Functional Morphology of Central and Peripheral Nervous System Department of General and Special Morphology 12 Akademika Pavlova St, 197376, St. Petersburg

参考

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