DYNAMICS OF STRUCTURAL CHANGES AND CD68 AND KI-67 MARKER EXPRESSION IN RAT THYMUS AFTER A SINGLE EXPOSURE TO IONIZING RADIATION



如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Objective - to examine the dynamics of structural changes and CD68 and Ki-67 marker expression in the thymus of rats at different time intervals after a single total irradiation in sublethal dose. Material and methods. In 30 rats after 3, 7, 14 and 30 days after experimental irradiation at a dose of 5 Gy, thymus was studied using histological, morphometric and immunohistochemical methods (Ki-67 and CD68 markers). Results. On the 3rd day after irradiation, the «inversion» of the thymus layers was detected with a decrease in the relative cortical area (by 15.1% as compared with data in controls) and a depletion of cell populations. Simultaneously, a significant increase in the expression of Ki-67 and CD68 markers in the subcapsular area was noted (by 71.3% 242.1% in comparison with the control, respectively). In subsequent time points of the experiment, a gradual recovery of thymus histoarchitectonics and cell populations was found that was due to the proliferative activity of thymocytes in the subcapsular zone at a constant level of expression of CD68 marker. Conclusions. The greatest expression of the morphologic changes in the thymus took place at 3 and 7 days after irradiation, with the restoration of histoarchitectonics and cell populations on the 30th day of the experiment.

全文:

受限制的访问

作者简介

M. Kriventsov

V.I. Vernadskiy Crimean Federal University

Email: maksimkgmu@mail.ru
Department of Normal Anatomy, S. I. Georgievsky Medical Academy Simferopol, 5/7 Lenina boulevard, Simferopol, Crimea 295000

V. Pikalyuk

V.I. Vernadskiy Crimean Federal University

Department of Normal Anatomy, S. I. Georgievsky Medical Academy Simferopol, 5/7 Lenina boulevard, Simferopol, Crimea 295000

G. Moroz

V.I. Vernadskiy Crimean Federal University

Department of Normal Anatomy, S. I. Georgievsky Medical Academy Simferopol, 5/7 Lenina boulevard, Simferopol, Crimea 295000

S. Kutya

V.I. Vernadskiy Crimean Federal University

Department of Normal Anatomy, S. I. Georgievsky Medical Academy Simferopol, 5/7 Lenina boulevard, Simferopol, Crimea 295000

参考

  1. Бибик Е. Ю., Мелещенко А. В., Ткаченко Е. В. и др. Влияние хронического воздействия ионизирующего излучения на морфогенез тимуса и красного костного мозга в эксперименте // Українськ. морфол. альманах. 2014. Т. 12, № 2. С. 7-9.
  2. Кривенцов М. А., Куцая В. В. Динамика структурных преобразований лимфатических узлов крыс после однократного воздействия ионизирующего фотонного излучения // Морфология. 2014. Т. 8, № 1. С. 53-57.
  3. Митин А. Н., Комогорова В. В., Литвина М. М. и др. Динамика субпопуляций тимоцитов при регенерации тимуса после облучения // Иммунология. 2012. № 6. С. 297-302.
  4. Мороз Г. А. Строение тимуса интактных крыс-самцов линии Вистар разного возраста // Свiт медицини та бiологii. 2009. Т. 5, № 3-2. С. 98-102.
  5. Орлов А. И. Непараметрические критерии согласия Колмогорова-Смирнова, омега-квадрат и ошибки при их применении // Науч. журн. КубГАУ. 2014. № 97 (03). С. 1-29.
  6. Старская И. С., Кудрявцев И. В., Полевщиков А. В. Морфологический анализ и динамика гидрокортизон-индуцированной атрофии тимуса // Мед. акад. журн. 2012. Т. 12, № 3. С. 94-96.
  7. Elmore S. A. Enhanced histopathology of the thymus // Toxicol. Pathol. 2006. Vol. 34, № 5. P. 656-665.
  8. Jinbo L., Hongquan C., Jianliang J. et al. X-ray irradiation selec tively kills thymocytes of different stages and impairs the maturation of donor-derived CD4+ CD8+ thymocytes in recipient thymus // J. Biomed. Res. 2012. Vol. 26, №5. P. 355-364.
  9. Mavragani I. V., Laskaratou D. A., Frey B. et al. Key mechanisms involved in ionizing radiation-induced systemic effects. A current review // Toxicol Res. 2015. Vol. 21, № 5 (1). P. 12-33.
  10. Pearse G. Normal structure, function and histology of the thymus // Toxicol. Pathol. 2006. Vol. 34, № 5. P. 504-514.
  11. Schneider C. A., Rasband W. S., Eliceiri K. W. NIH Image to ImageJ: 25 years of image analysis // Nature Methods. 2012. № 9. P. 671-675.
  12. Williams J. P., Brown S. L., Georges G. E. et al. Animal models for medical countermeasures to radiation exposure // Radiat Res. 2010. Vol. 173 (4). P. 557-578.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Eco-Vector, 2017


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.
##common.cookie##