IMPROVED METHOD FOR THE DEMONSTRATION OF THE CRYSTALLINE LENS CELLULAR STRUCTURES
- 作者: Korsakova NV1, Sergeeva VE1, Korsakova NV1, Sergeyeva VY.1
-
隶属关系:
- 期: 卷 140, 编号 4 (2011)
- 页面: 65-66
- 栏目: Articles
- ##submission.dateSubmitted##: 09.05.2023
- ##submission.datePublished##: 15.11.2011
- URL: https://j-morphology.com/1026-3543/article/view/399512
- DOI: https://doi.org/10.17816/morph.399512
- ID: 399512
如何引用文章
详细
A new method for the demonstration of the lens epithelial cells and fibers in the eye of the experimental animals (rats) is described. The method proposed is not laborious and is simple in utilization. It is based on the intravital administration of methylene blue solution to the animal with the subsequent supravital crystalline lens staining at 37ºC. The material was then embedded into paraffin, and the sections were stained with hematoxylin-eosin. The sections obtained using the proposed method, give an opportunity for the detailed demonstration of the morphological features of the lens cells.
参考
- Катаракта. Под ред. З.Ф. Веселовской. Киев, Книга плюс, 2002.
- Churchill G.C. and Louis CF. Stimulation of P2U purinergic or ά1-adrenergic receptors mobilizes Ca2+ in lens cells. Invest. Ophthalmol. Vis. Sci., 1997, v. 38, № 5, p. 855-865.
- Collison D.J. and Duncan G. Regional differences in functional receptor distribution and calcium mobilization in the intact human lens. Invest. Ophthalmol. Vis. Sci., 2001, v. 42, № 10, p. 2355-2363.
- Duncan G. and Collison D.J. Role of the non-neuronal cholinergic system in the eye: a review. Life Sci., 2003, v. 72, № 18-19, p. 2013-2009.
- Hernandez C.M. Cataracts: Сauses, Symptoms, and Surgery. New York, Nova Publishers, 2010.