Email this article IMPROVED METHOD FOR THE DEMONSTRATION OF THE CRYSTALLINE LENS CELLULAR STRUCTURES
- Authors: Korsakova NV1, Sergeeva VE1, Korsakova NV1, Sergeyeva VY.1
-
Affiliations:
- Issue: Vol 140, No 4 (2011)
- Pages: 65-66
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/399512
- DOI: https://doi.org/10.17816/morph.399512
- ID: 399512
Cite item
Open Access
Access granted
Subscription or Fee Access
Abstract
A new method for the demonstration of the lens epithelial cells and fibers in the eye of the experimental animals (rats) is described. The method proposed is not laborious and is simple in utilization. It is based on the intravital administration of methylene blue solution to the animal with the subsequent supravital crystalline lens staining at 37ºC. The material was then embedded into paraffin, and the sections were stained with hematoxylin-eosin. The sections obtained using the proposed method, give an opportunity for the detailed demonstration of the morphological features of the lens cells.
Keywords
References
- Катаракта. Под ред. З.Ф. Веселовской. Киев, Книга плюс, 2002.
- Churchill G.C. and Louis CF. Stimulation of P2U purinergic or ά1-adrenergic receptors mobilizes Ca2+ in lens cells. Invest. Ophthalmol. Vis. Sci., 1997, v. 38, № 5, p. 855-865.
- Collison D.J. and Duncan G. Regional differences in functional receptor distribution and calcium mobilization in the intact human lens. Invest. Ophthalmol. Vis. Sci., 2001, v. 42, № 10, p. 2355-2363.
- Duncan G. and Collison D.J. Role of the non-neuronal cholinergic system in the eye: a review. Life Sci., 2003, v. 72, № 18-19, p. 2013-2009.
- Hernandez C.M. Cataracts: Сauses, Symptoms, and Surgery. New York, Nova Publishers, 2010.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).