血液凝固对ex vivo血细胞免疫反应性的影响

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详细

论证。止血系统与免疫系统之间的相互作用保障着人体对外部病原体的防御。然而，血液凝固对免疫细胞反应性的影响研究尚不充分。

目的 — 研究血液凝固对其ex vivo免疫发应特性的影响。

材料和方法。献血者的血液样本与肝素一起培养（用于血浆的研究）或不与肝素一起培养（用于血清的研究）。在加入过氧化氢或者臭氧生理溶液后，通过化学发光强度评估了血浆和血清的抗氧化活性。用脂多糖（LPS）培养血液3或18小时，利用免疫酶法测定了细胞因子的含量。

结果。血清的抗氧化活性明显高于血浆。血液凝固过程明显减少了血细胞自发分泌和 LPS 诱导的肿瘤坏死因子TNF-α，但没有实质性影响白细胞介素IL-1、IL-6、IL-8 和 C反应蛋白的分泌。另一方面，这个过程导致了血细胞自发分泌和LPS诱导的血管内皮生长因子VEGF分泌增加。 含有LPS的血清样本也检测到了降钙素原的明显增加。

结论。血液凝固可增强血液的抗氧化性，减弱免疫活性细胞的炎症活动，从而促进再生过程的发展。

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作者简介

Anatoly A. Pyshenko

Petrovsky National Research Center of Surgery

Email: anatoliy.dr@yandex.ru
ORCID iD: 0009-0002-1117-608X
SPIN 代码: 8973-0238
俄罗斯联邦, Moscow

Tatiana Ya. Lyubavskaya

Petrovsky National Research Center of Surgery

Email: rnc2016@mail.ru
ORCID iD: 0009-0002-8106-8148
SPIN 代码: 1434-2924

Cand. Sci. (Biology)

俄罗斯联邦, Moscow

Irina A. Seledtsova

Petrovsky National Research Center of Surgery

Email: iax34@yandex.ru
ORCID iD: 0009-0006-0401-1876
SPIN 代码: 7001-6428

MD, Cand. Sci. (Medicine)

俄罗斯联邦, Moscow

Viktor I. Seledtsov

Petrovsky National Research Center of Surgery

编辑信件的主要联系方式.
Email: seledtsov@rambler.ru
ORCID iD: 0000-0002-4746-8853
SPIN 代码: 6469-9230

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Moscow

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2. Fig. 1. Chemiluminescence intensity (CI) of plasma and serum samples in the presence of 0.35 M hydrogen peroxide (a) and saline containing 1 µg/ml of ozone (b). kPPS, total number of impulses in the photon detection mode. The control values of chemiluminescence intensity of the used solutions of hydrogen peroxide and ozonated saline were 61,870 and 2,850, respectively. Representative results with plasma and serum samples obtained from 4 donors are presented.

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3. Fig. 2. Content of bioactive molecules in plasma (yellow columns) and serum (red columns) after incubation of whole blood with LPS for 3 and 18 h: a, IL-1 (n = 5); b, IL-6 (n = 5); c, IL-8 (n = 5); d, TNF-α (n = 7, *, statistically significant difference from the corresponding plasma sample, p < 0.02); e, C-reactive protein (n = 7); f, procalcitonin (n = 7, *, statistically significant difference from the corresponding plasma sample, p < 0.05); g, VEGF (n = 7, *, statistically significant difference from the corresponding plasma sample, p < 0.03). Data are presented as mean and error of mean, n is the number of samples (blood donors).

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4. Fig. 3. IL-8 (a) and VEGF (b) content in plasma and serum during the first hours of whole blood incubation. Representative results of experiments with blood samples obtained from 2 donors are presented.

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