Email this article DISTRIBUTION OF LIPOFUSCIN IN HUMAN CEREBELLAR CORTEX
- Authors: Sukhorukova Y.G.1, Gilerovich Y.G.1, Syrtsova M.A.1, Novikova A.D.1, Korzhevskiy D.E.1
-
Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 150, No 6 (2016)
- Pages: 25-28
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/397791
- DOI: https://doi.org/10.17816/morph.397791
- ID: 397791
Cite item
Open Access
Access granted
Abstract
Lipofuscin is a recognized intracellular marker of aging. The purpose of this study was to establish the topographic pattern of distribution of lipofuscin granule clusters in the cerebellar cortex. With the use of fluorescence microscopy The pigment was demonstrated due to its property of autofluorescence, in the sections of human cerebellar cortex (n=25, age 20-89 years). The analysis of preparations showed the presence of lipofuscin granules, predominantly with yellow-green autofluorescence, in different layers of the cerebellar cortex. A tendency of these granules to increase in number and sizes with age was detected. Lipofuscin was found to accumulate in the layers of cerebellar cortex unevenly. Most pronounced lipofuscin clusters were seen in the Purkinje cells, where a shift of its intracellular localization from perinuclear to the site of the processes’ origin was observed.
Keywords
Full Text
About the authors
Ye. G. Sukhorukova
Institute of Experimental Medicine
Email: len48@inbox.ru
Ye. G. Gilerovich
Institute of Experimental Medicine
M. A. Syrtsova
Institute of Experimental Medicine
A. D. Novikova
Institute of Experimental Medicine
D. E. Korzhevskiy
Institute of Experimental Medicine
Email: dek2@yandex.ru
References
- Гилерович Е. Г., Федорова Е. А., Григорьев И. П., Коржевский Д. Э. Морфологические основы реорганизации коры мозжечка крыс при старении // Журн. эвол. биохим. 2015. Т. 15, № 5. С. 370-376.
- Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г. и др. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии // Морфология. 2013. Т. 143, вып. 2. С. 81-85.
- Лилли Р. Патогистологическая техника и практическая гистохимия. М.: Мир, 1969.
- Bennett I. J., Madden D. J. Disconnected aging: cerebral white matter integrity and age-related differences in cognition // Neuroscience. 2014. Vol. 276. P. 187-205.
- Bernard J. A., Seidler R. D. Moving forward: age effects on the cere bellum underlie cognitive and motor declines // Neurosci. Biobehav. Rev. 2014. № 42. P. 193-207.
- Bertoni-Freddari C., Fattoretti P., Giorgetti B. et al. Decay of mitochondrial metabolic competence in the aging cerebellum // Ann N Y Acad. Sci. 2004. Vol. 1019. P. 29-32.
- Brizzee K. R., Cancilla P.A., Sherwood N., Timiras P.S. The amount and distribution of pigments in neurons and glia of the ce rebral cortex. Autofluorescent and ultrastructural studies // J. Gerontol. 1969. Vol. 24, № 2. P. 127-135.
- Brunk U. T., Terman A. Lipofuscin: mechanisms of age-related accumulation and influence on cell function // Free Radic. Biol. Med. 2002. Vol. 33, № 5. P. 611-619.
- Glees P., Aloj Totaro E., Pisanti F. Possible removal route of osmiophilic material (lipofuscin) from spinal ganglia of Torpedo marmorata // J. Hirnforsch. 1986. Vol. 27, № 1. P. 79-86.
- Goyal V. K. Lipofuscin pigment accumulation in human brain during aging // Exp. Gerontol. 1982. Vol. 17, № 6. P. 481-487.
- Koppelmans V., Hirsiger S., Mйrillat S. et al. Cerebellar gray and white matter volume and their relation with age and manual motor performance in healthy older adults // Hum. Brain Mapp. 2015. Vol. 36, № 6. P. 2352-2363.
- Koziol L. F., Budding D., Andreasen N. et al. Consensus paper: the cerebellum’s role in movement and cognition // Cerebellum. 2014. Vol. 13, № 1. P. 151-177.
- Manto M., Bower J. M., Conforto A. B. et al. Consensus paper: roles of the cerebellum in motor control - the diversity of ideas on cerebellar involvement in movement // Cerebellum. 2012. Vol. 11, № 2. P. 457-487.
- Schmahmann J. D., Caplan D. Cognition, emotion and the cerebellum // Brain. 2006. Vol. 129, № 2. P. 290-292.
- Terman A., Brunk U. T. Lipofuscin // Int. J. Biochem. Cell Biol. 2004. Vol. 36, № 8. P. 1400-1404.
- Zhang C., Zhu Q., Hua T. Aging of cerebellar Purkinje cells // Cell Tissue Res. 2010. Vol. 341, № 3. P. 341-347.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).