NEUROGLOBIN IN CEREBELLAR PURKINJE CELLS OF RATS WITH CHOLESTASIS

Objective - to estimate the content of neuroglobin in cerebellar Purkinje cells in the dynamics of experimental cholestasis in rats. Materials and methods. The study was performed on the cerebellar cortex paraffin sections processed for immunohistochemical detection of neuroglobin in Purkinje neurons, obtained from 60 outbred albino male rats, weighing 200-250 g, after an experimental intervention. Experimental animals were subjected to the common bile duct ligation; control animals underwent sham operation that did not prevent the physiological bile outflow into the duodenum. Results. It was found that after the common bile duct ligation, the content of neuroglobin in the cerebellar Purkinje cell perikarya fluctuated in a wave-like manner: it was significantly reduced at days 2-20 of the experiment (with the minimum at days 5-10) under conditions of cholestasis, and increased at days 45-90, after cholestasis self-healing (with the maximum at day 45). Conclusions. After the common bile duct ligation, the neuroglobin expression in the cerebellar Purkinje cell perikarya fluctuated in a wave-like manner: it decreased significantly in cholestasis and increased after its self-healing.

cerebellum, Purkinje cells, neuroglobin, cholestasis, rats