VIMENTIN AND S100 PROTEIN IN THE DEVELOPING RAT DORSAL ROOT GANGLION CELLS
- Authors: Kolos Y.A.1, Korzhevskiy D.E.1
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Affiliations:
- RAMS North-Western Branch Institute of Experimental Medicine
- Issue: Vol 143, No 3 (2013)
- Pages: 074-076
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398639
- DOI: https://doi.org/10.17816/morph.398639
- ID: 398639
Cite item
Abstract
The aim of this research was to study the expression of vimentin, one of the intermediate filament proteins, and S100 protein in the cells of developing dorsal root ganglion in prenatal ontogenesis in Wistar rat embryos (n=22) on 12–19 days of development and in newborn rats (n=6). Immunohistochemical staining methods were used to study the formation of rat dorsal root ganglion glial cells. Vimentin-immunopositive neural and glial progenitor cells were present in dorsal root ganglion primordia in rat embryos at 12–17 prenatal days. At the later stages (day 19 of prenatal life and day 1 of postnatal life) vimentin was found to be synthesized only by the differentiating glial cells. It was shown that at the same time, S100 protein was first detected in the glial cells of rat dorsal root ganglion. On postnatal day 1, the tendency was noted for the accumulation of S100 protein in the larger neurons, that was characteristic to mature dorsal root ganglia.
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About the authors
Ye. A. Kolos
RAMS North-Western Branch Institute of Experimental Medicine
D. E. Korzhevskiy
RAMS North-Western Branch Institute of Experimental Medicine
Email: iemmorphol@yandex.ru
References
- Кнорре А. Г. Эмбриональный гистогенез. Л., Медицина, 1971.
- Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г. и др. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуногистохимических исследований и конфокальной лазерной микроскопии. Морфология, 2013, т. 143, вып. 2, с. 81–85.
- Ноздрачев А. Д. и Чумасов Е. И. Периферическая нервная система. СПб., Наука, 1999.
- Boisseau S. and Simonneau M. Mammalian neuronal differentiation: early expression of a neuronal phenotype from mouse neural crest cells in a chemically defined culture. Development, 1989, v. 106, № 4, p. 665–674.
- Kishi M., Tanabe J., Schmelzer J. D. and Low P. A. Morphometry of dorsal root ganglion in chronic experimental diabetic neuropathy. Diabetes, 2002, v. 51, № 3, р. 819–824.
- Nascimento R. S., Santiago M. F., Marques S. A. et al. Diversity among satellite glial cells in dorsal root ganglia of the rat. Braz. J. Med. Biol. Res., 2008, v. 41, № 11, р. 1011–1017.
- Sapunar D., Kostic S., Banozic A. and Puljak L. Dorsal root gang lion — a potential new therapeutic target for neuropathic pain. J. Pain. Res., 2012, № 5, р. 31–38.