Email this article DECALCIFICATION IN FORMIC ACID AFTER FIXATION IN ZINC-ETHANOLFORMALDEHYDE DOES NOT PRECLUDE IDENTIFICATION OF NEURONAL AND GLIAL MARKERS
- Authors: Kolos E.A.1, Korzhevskiy D.E.1
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Affiliations:
- RAMS North-Western Branch Institute of Experimental Medicine
- Issue: Vol 144, No 4 (2013)
- Pages: 076-079
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398706
- DOI: https://doi.org/10.17816/morph.398706
- ID: 398706
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Abstract
The aim of this study was to determine the possibility of immunohistochemical demonstration of various tissue antigens in paraffin sections of zinc-ethanol-formaldehyde-fixed spinal cord, surrounded by the vertebral tissues and adjacent muscles, after decalcification in formic acid, in adult (n=5) and newborn (n=2) Wistar rats. In the present work, the antibodies used were against: glial fibrillary acidic protein, neurofilaments, neuronal specific nuclear protein, enzyme tyrosine hydroxylase, protein of synaptic vesicles — synaptophysin and the intermediate filament protein — vimentin. Combination of fixation in zinc-ethanolformaldehyde and decalcification in 21% formic acid solution was shown to provide an optimal preservation of the antigens studied.
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About the authors
E. A. Kolos
RAMS North-Western Branch Institute of Experimental Medicine
D. E. Korzhevskiy
RAMS North-Western Branch Institute of Experimental Medicine
Email: iemmorphol@yandex.ru
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).