Email this article HISTOMORPHOMETRIC CHARACTHERISTIC OF SKELETAL MUSCLE REGENERATING AFTER A CLOSED PARTIAL CRUSH INJURY
- Authors: Shchudlo N.A1, Shchudlo M.M.1, Kononovich N.A.1
-
Affiliations:
- G. A. Ilizarov Russian Scientific Center for Restorative Traumatology and Orthopedics
- Issue: Vol 146, No 4 (2014)
- Pages: 59-63
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398773
- DOI: https://doi.org/10.17816/morph.398773
- ID: 398773
Cite item
Open Access
Access granted
Abstract
To evaluate the degree of skeletal muscle regeneration in 15 adult Wistar rats, the closed partial crush injury of anterior tibial muscle was modeled. The study was performed using the methods of light microscopy and computer-assisted morphometry of semithin sections. The continuity of the crushed muscle fibres (MF) was restored by day 21 after the injury. After 90 days endomysial fibrosis was substantially reduced and its vasculairity increased; the variability of MF diameters was restored, however the branched structure and myopathic changes of the regenerated tissue persisted and were presumably associated with an incomplete reinnervation of the damaged intramuscular nerves.
Keywords
Full Text
About the authors
N. A Shchudlo
G. A. Ilizarov Russian Scientific Center for Restorative Traumatology and OrthopedicsClinical-and-Experimental Laboratory of Reconstructive-and-Restorative Microsurgery and Surgery of the Hand
M. M. Shchudlo
G. A. Ilizarov Russian Scientific Center for Restorative Traumatology and Orthopedics
Email: m.m.sch@mail.ru
Clinical-and-Experimental Laboratory of Reconstructive-and-Restorative Microsurgery and Surgery of the Hand
N. A. Kononovich
G. A. Ilizarov Russian Scientific Center for Restorative Traumatology and OrthopedicsClinical-and-Experimental Laboratory of Reconstructive-and-Restorative Microsurgery and Surgery of the Hand
References
- Булякова Н. В. и Азарова В. С. Возрастные особенности регенерации мышц при травме, воздействии He-Ne лазера и аллопластике мышечной тканью от животного того же возраста. Морфология, 2013, т. 143, № 3, с. 45-49.
- Данилов Р. К. Раневой процесс: гистогенетические основы. СПб., Изд-во ВмедА им. С. М. Кирова, 2008.
- Европейская конвенция по защите позвоночных животных, используемых для экспериментальных и других научных целей. Вопр. реконструкт. пластич. хир., 2003, №4, c. 34-36.
- Щудло М. М., Ступина Т. А., Щудло Н. А. Количественный анализ метахромазии суставного хряща в телепатологии. Известия Челябинского НЦ (УРО РАН), 2004, № 25 (Спец. вып.), с. 17-22.
- Уикли Б. Электронная микроскопия для начинающих. М., Мир, 1975.
- Bassaglia Y., Gautron J. Fast and slow rat muscles degenerate and regenerate differently after whole crush injury. J. Muscle Res. Cell Motil., 1995, v. 16, № 4, p. 420-429.
- Charge S. B., Rudnicki M. A. Cellular and molecular regulation of muscle regeneration. Physiol. Rev., 2004, v. 84, p. 209-238.
- Crassous B., Richard-Bulteau H., Deldicque L. et al. Lack of effects of creatine on the regeneration of soleus muscle after injury in rats. Med. Sci. Sports Exerc., 2009, v. 41, № 9, p. 1761-1769.
- Díaz-Flores L., Gutiérrez R., Madrid J. F. et al. Pericytes. Morpho function, interactions and pathology in a quiescent and activated mesenchymal cell niche. Histol. Histopathol., 2009, v. 24, № 7, p. 909-969.
- Fink E., Fortin D., Serrurier B. et al. Recovery of contractile and metabolic phenotypes in regenerating slow muscle after notexininduced or crush injury. J. Muscle Res. Cell Motil., 2003, v. 24, № 7, p. 421-429.
- Harry L.E., Sandison A., Paleolog E. M. et al. Comparison of the healing of open tibial fractures covered with either muscle or fasciocutaneous tissue in a murine model. J. Orthop Res., 2008, v. 26, № 9, p. 1238-1244.
- Kirkendall D. T. and Garrett W. E. Jr. Clinical perspectives regarding eccentric muscle injury. Clin. Orthop. Relat. Res., 2002, v. 403 (Suppl.), p. S81-S89.
- Negishi S., Li Y., Usas A. et al. The effect of rela xin treatment on skeletal muscle injuries. Am. J. Sports Med., 2005, v. 33, № 12, p. 1816-1824.
- Ontell M. Muscle satellite cells: a validated technique for light microscopic identification and a quantitative study of changes in their population following denervation. Anat. Rec.,1974, v. 178, p. 211-228.
- Tsuburaya R., Suzuki T., Saiki K. et al. Lobulated fibers in a patient with 46-year history of limb-girdle muscle weakness. Neuropathology, 2011, v. 31, I. 4, p. 455-457.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).