Email this article CHANGES IN THE EXPRESSION OF BCL-2 ANTIAPOPTOTIC PROTEIN IN RAT NEOCORTEX AND HIPPOCAMPUS UNDER THE INFLUENCE OF VARIOUS MODES OF HYPOBARIC HYPOXIA
- Authors: Churilova A.V.1, Glushchenko T.S.1, Samoilov M.O.1
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Affiliations:
- RAS I. P.Pavlov Institute of Physiology
- Issue: Vol 146, No 5 (2014)
- Pages: 7-13
- Section: Articles
- URL: https://j-morphology.com/1026-3543/article/view/398774
- DOI: https://doi.org/10.17816/morph.398774
- ID: 398774
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Abstract
Using immunocytochemical method, the level of expression of Bcl-2 antiapoptotic factor was studied in neurons of the neocortex and hippocampus in 72 male Wistar rats exposed to damaging severe hypoxia (SH), moderate hypobaric hypoxia (MHH), as well as their combination. After SH (180 mmHg) Bcl-2 expression in the neurons of the brain regions examined was reduced or unchanged. The effect of preconditioning with one trial of MHH (360 mmHg) on Bcl-2 expression was similar to that seen after SH. In contrast, preconditioning with repeated exposures to MHH significantly up-regulated Bcl-2 expression levels 3-24 h after SH that apparently protected neurons from SH-induced injury. MHH alone, not followed by SH, significantly increased Bcl-2 expression only after multiple (three or six) exposures whereas single MHH exposure had no effect on Bcl content. Hence, up-regulation of Bcl-2 seen in response to multiple MHH trials appears to be important for the formation of the mechanisms of brain neuronal tolerance to damaging factors.
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About the authors
A. V. Churilova
RAS I. P.Pavlov Institute of Physiology
Email: annch05@mail.ru
Laboratory of Regulation of Brain Neuron Functions
T. S. Glushchenko
RAS I. P.Pavlov Institute of Physiology
Email: tsgluschenko@mail.ru
Laboratory of Regulation of Brain Neuron Functions
M. O. Samoilov
RAS I. P.Pavlov Institute of Physiology
Email: samoilov@pavlov.infran.ru
Laboratory of Regulation of Brain Neuron Functions
References
- Самойлов М. О. и Рыбникова Е. А. Молекулярно-клеточные и гормональные механизмы индуцированной толерантности мозга к экстремальным факторам среды. Рос. физиол. журн. им. И. М. Сеченова, 2012, т. 98, № 1, с. 108-126.
- Самойлов М. О., Рыбникова Е. А. и Чурилова А. В. Сигнальные молекулярные и гормональные механизмы формирования протективных эффектов гипоксического прекондиционирования. Пат. физиол., 2012, № 3, с. 3-10.
- Чурилова А. В., Глущенко Т. С. и Самойлов М. О. Изменения нейронов гиппокампа и неокортекса крыс под влиянием различных режимов гипобарической гипоксии. Морфология, 2012, т. 141, вып. 1, с. 7-11.
- Fujimura M., Morita-Fujimura Y., Noshita N. et al. The cytosolic antioxidant copper/zinc-superoxide dismutase prevents the early release of mitochondrial cytochrome c in ischemic brain after transient focal cerebral ischemia in mice. J. Neurosci., 2000,v. 20, p. 2817-2824.
- Gillies L. A. and Kuwana T. Apoptosis regulation at the mitochondrial outer membrane. J. Cell. Biochem., 2014, v. 115, № 4, p. 632-640.
- Hardwick J. M., Chen Y.B. and Jonas E. A. Multipolar functions of BCL-2 proteins link energetics to apoptosis. Trends Cell Biol., 2012, v. 22, p. 318-328.
- Kirino T. Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res., 1982, v. 239, p. 57-69.
- Love S. Apoptosis and brain ischemia. Prog. Neuro psychopharmacol. Biol. Psychiatry, 2003, v. 27, p. 267-282.
- Mayer B. and Oberbauer R. Mitochondrial regulation of apoptosis. News Physiol. Sci., 2003, v. 18, p. 89-94.
- Ouyang Y. B. and Giffard R. G. Cellular neuroprotective mechanisms in cerebral ischemia: Bcl-2 family proteins and protection of mitochondrial function. Cell Calcium, 2004, v. 36, p. 303-311.
- Ouyang Y. B., Stary C. M., Yang G. Y. and Giffard R. Micro RNAs: innovative rargets for cerebral ischemia and stroke. Curr. Drug Targets, 2013, v. 14, № 1, p. 90-101.
- Rybnikova E., Glushchenko T., Churilova A. et al. Expression of glucocorticoid and mineralocorticoid receptors in hippocampus of rats exposed to various modes of hypobaric hypoxia: Putative role in hypoxic preconditioning. Brain Res., 2011, v. 1381, p. 66-77.
- Rybnikova E., Gluschenko T., Tulkova E. et al. Preconditioning induces prolonged expression of transcription factors pCREB and NF-kappa B in the neocortex of rats before and following severe hypobaric hypoxia. J. Neurochem., 2008, v. 106, № 3, p. 1450-1458.
- Rybnikova E., Sitnik N., Gluschenko T. et al. The preconditioning modified neuronal expression of apoptosis-related proteins of Bcl-2 superfamily following severe hypobaric hypoxia in rats. Brain Res., 2006, v. 1089, p. 195-202.
- Rybnikova E., Vataeva L., Tyulkova E. et al. Preconditioning prevents impairment of passive avoidance learning and suppression of brain NGFI-A expression induced by severe hypoxia. Behav. Brain Res., 2005, v. 160, p. 107-114.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).