CYTOPLASMIC LOCALIZATION OF NEUN/Fox-3 NUCLEAR PROTEIN IN THE PANCREATIC CELLS



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Abstract

The study of the sections of Wistar rat pancreas (n=4) using the methods of immunocytochemistry and confocal laser microscopy has demonstrated previously unknown fact of the presence of NeuN protein in the secretory epithelial cells of the exocrine part of this organ. The protein was located in the apical part of the cytoplasm slightly below the zone of zymogen granules. Until now the nuclear protein NeuN was considered to be the panneuronal differentiation marker and was used in the studies of neurogenesis.

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About the authors

D. E. Korzhevskiy

RAS Research Institute of Experimental Medicine; St.Petersburg State University

Email: dek2@yandex.ru
Department of Fundamental Problems of Medicine and Medical Technology

G. V. Beznin

RAS Research Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

Ye. A. Kolos

RAS Research Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

O. V. Kirik

RAS Research Institute of Experimental Medicine

Email: iemmorphol@yandex.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology

References

  1. Коржевский Д. Э., Петрова Е. С., Кирик О. В. и др. Нейральные маркеры, используемые при изучении дифференцировки стволовых клеток // Клеточная трансплантология и тканевая инженерия. 2010. Т. 5, вып. 3. С. 57-63.
  2. Сухорукова Е. Г. Ядерный белок NeuN в нейронах черного вещества головного мозга человека // Морфология. 2013. Т. 143, вып. 2. С. 78-80.
  3. Чумасов Е. И., Петрова Е. С., Коржевский Д. Э. Распределение и структурная организация автономных нервных аппаратов в поджелудочной железе крысы (иммуногистохимическое исследование) // Морфология. 2011. Т. 139, вып. 3. С. 51-58.
  4. Darnell R. B. RNA Protein Interaction in Neurons // Annu. Rev. Neurosci. 2013. Vol. 36. P. 243-270.
  5. Dent M. A., Segura-Anaya E., Alva-Medina J., Aranda-Anzaldo A. NeuN/Fox-3 is an intrinsic component of the neuronal nuclear matrix // FEBS Lett. 2010. Vol. 584, № 13. P. 2767-2771.
  6. Kim K. K., Adelstein R. S., Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors // J. Biol Chem. 2009. Vol. 284. P. 31052-31061.
  7. Mullen R. J., Buck C. R., Smith A. M. NeuN, a neuronal specific nuclear protein in vertebrates // Development. 1992. Vol. 116. P. 201-211.

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