ANALYSIS OF MICRONUCLEI FREQUENCY IN EPITHELIAL CELLS AND LYMPHOCYTES AND ITS CORRELATION WITH GLUTATHIONE-S-TRANSFERASE GENE POLYMORPHISM IN PATIENTS WITH TICK-BORNE ENCEPHALITIS



如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

Objective - to study the changes in the frequency of micro-nucleated peripheral blood lymphocytes and buccal epithelial cells in patients with tick-borne encephalitis (TBE) during the acute and convalescent periods of the disease and their correlation with variants of glutathione-S-transferase ( GSTM1 or GSTT1 ) genes in the patient’s genotype. Material and methods. The study included 54 patients with TBE and 35 healthy (control) residents of the Tomsk and Tyumen regions. The enrolled participants were examined by clinical, laboratory and cytogenetic methods. The latter included micronucleus assay in buccal cells and peripheral blood cytokinesis-blocked lymphocytes. The material for the study (peripheral blood and buccal cell smears) was obtained repeatedly during the admission of patients to treatment, and after 1 week, 1, 3 and 6 months. The allele variants of the GSTM1 and GSTT1 genes were detected by polymerase chain reaction. Results The study demonstrated a significant increase in the frequency of micronucleated cells in TBE patients compared with the control group. The most significant and the most prolonged increase in the frequency of micronucleated cells was associated with the mutant inactive alleles of the GSTM1 (0/0) and GSTT1 (0/0) genes. Conclusion. If a TBE patient was a carrier of the mutant inactive alleles of GSTM1 (0/0) and GSTT 1(0/0) genes, the cytogenetic instability in T lymphocytes of the peripheral blood could persist for half a year. Key words: buccal epithelium, cytokinesis-blocked lymphocytes, glutathione-S-transferase, micronucleus analysis, tick-borne encephalitis

全文:

受限制的访问

作者简介

O. Anan’eva

Tyumen State Medical University

Email: olvasan@mail.ru
Department of Clinical Laboratory Diagnostics 54 Odesskaya St., Tyumen 625023

N. Ilyinskikh

National Research Tomsk State University; Siberian State Medical University

Email: nauka-tomsk@yandex.ru
Department of Ecology, Environmental Sciences and Environmental Engineering; Department of Biology and Genetics 36 Lenin Avenue, Tomsk 634050; 2 Moscow tract, Tomsk 634050

E. Ilyinskikh

National Research Tomsk State University; Siberian State Medical University

Department of Ecology, Environmental Sciences and Environmental Engineering; Department of Biology and Genetics 36 Lenin Avenue, Tomsk 634050; 2 Moscow tract, Tomsk 634050

E. Zamyatina

Siberian State Medical University

Email: infconf2009@mail.ru
Department of Biology and Genetics 2 Moscow tract, Tomsk 634050

A. Durov

Tyumen State University; Tyumen State Medical University

Email: amdurov@mail.ru
Department of Physical Culture and Sport, Institute of Physical Education; Department of Biology and Genetics 6 Volodarskogo St., Tyumen 625003

参考

  1. Григорьева С. А., Никитина В. А., Косякова Н. В. Кириллов А. В., Аксенова М. Г., Сидорова И. Е., Ревазова Л. А., Чеботарев А. Н., Бочков Н. П. Частота полиморфизма генов ферментов биотрансформации ксенобиотиков CYP1A1, GSTM1 и GSTT1 у жителей города Москвы // Медицинская генетика. 2007. № 3. С. 38-43.
  2. Захарычева Т. А., Ковальский Ю. Г., Лебедько О. А., Мжельская Т. В. Оксидативный стресс у больных клещевым энцефалитом на Дальнем Востоке Российской Федерации // Дальневост. журн. инфекц. патологии. 2012. Т. 20, № 8. С. 41-45.
  3. Ильинских Н. Н., Васильев С. А., Кравцов В. Ю. Микроядерный тест в скрининге и мониторинге мутагенов. Saarbrucken (Deutschland): LAP LAMBERT Academic Publishen. GmbH&Co. KG., 2011. 216 с.
  4. Ильинских И. Н., Ильинских Е. Н., Новицкий В. В., Ильинских Н. Н., Ткаченко С. Б. Инфекционная кариопатология. Томск: Томский государственный университет, 2005. 168 с.
  5. Bolognesi C., Fenech M. Micronucleus assay in human cells: lymphocytes and buccal cells // Methods Mol Biol. 2013. Vol. 1044. P. 191-207. doi: 10.1007/978-1-62703-529-3_10
  6. Fenech M., Kirsch-Volders M., Natarajan A. T., Surralles J., Crott J. W., Parry J., Norppa H., Eastmond D. A., Tucker J. D., Thomas P. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells // Mutagenesis. 2011. Vol. 26, № 1. P. 125-132. doi: 10.1093/mutage/geq052
  7. Fernandes R. C., Hasan M., Gupta H., Geetha K., Rai P. S., Hande M. H., D’Souza S. C., Adhikari P., Brand A., Satya moorthy K. Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population // Mol. Genet. Genomics. 2015. Vol. 290, № 3. P. 1155-1168. doi: 10.1007/ s00438-014-0984-4
  8. Ilyinskikh N. N., Ilyinskikh I. N. Effects of virus of tick-borne encephalitis on the chromosome apparatus of human cells // Cytology and Genetics. 1976. Vol. 10, № 4. P. 331-333.
  9. Parsons M., Campa A., Lai S., Li Y., Martinez J. D., Murillo J., Greer P., Martinez S. S., Baum M. K. Effect of GSTM1-polymorphism on disease progression and oxidative stress in HIV infection: modulation by HIV/HCV co-infection and alcohol consumption // J. AIDS Clin. Res. 2013. Vol. 4, № 9. P. 248-252. doi: 10.4172/2155-6113.1000237
  10. Priya K., Yadav A., Kumar N., Gulati S., Aggarwal N., Gupta R. Glutathione S-transferase gene polymorphisms: modulator of genetic damage in gasoline pump workers // Int. J. Toxicol. 2015. Vol. 34, № 6. P. 500-504. doi: 10.1177/1091581815603935
  11. Saha S. K., Lee S. B., Won J., Choi H. Y., Kim K., Yang G. M., Dayem A. A., Cho S. G. Correlation between oxidative stress, nutrition, and cancer initiation // Int. J. Mol. Sci. 2017. Vol. 18, № 7. e1544. doi: 10.3390/ijms18071544

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Eco-Vector, 2018


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.
##common.cookie##