FORMATION OF GABA-ERGIC NEURAL NETWORK IN BÖTZINGER COMPLEX IN RATS DURING EARLY POSTNATAL PERIOD IN NORM AND IN PRENATAL DEFICIENCY OF ENDOGENOUS SEROTONIN
- Authors: Khozhai L.I.1, Ilyichova N.V.2
-
Affiliations:
- I. P.Pavlov RAS Institute of Physiology
- RAS Institute of Cytology
- Issue: Vol 150, No 4 (2016)
- Pages: 44-49
- Section: Articles
- Submitted: 09.05.2023
- Published: 15.08.2016
- URL: https://j-morphology.com/1026-3543/article/view/397738
- DOI: https://doi.org/10.17816/morph.397738
- ID: 397738
Cite item
Abstract
The dynamics of the distribution of GABAergic neurons and neurons expressing different types of GABA receptors (GABAAα1 and GABAB1) was studied in Bötzingercomplex (BötC) in the early postnatal period (the period of functional maturation of the respiratory system in mammals) in norm and prenatal reduction of serotonin content in Wistar rats. The brain was studied on postnatal Days 5, 9 and 20 in two groups of rat pups: control (n=9), born by intact females, and experimental (n=13), born from mothers that received parachlorophenylalanine, causing the depression of endogenous serotonin level. Imunocytochemical methods were used to detect the neurons producing GABA and expressing GABA and GABA Aα1B1 receptors. It was shown that the maturation of the inhibitory GABAergic network in BötC occurred in the early postnatal period (by Day 9). Simultaneously with GABA, the expression of GABA and GABA Aα1B1 receptors took place, however their maturation has the distinctive features. The formation of GABAAα1 receptors occurred earlier (by Day 9) and coincided in time with the expression of GABA. The maturation of GABAB1 receptors happened later - only by the third week. Prenatal serotonin deficiency caused a delay in the expression of GABA and GABAAα1 receptors by the neurons of BötC, as well as the disruption of the formation of a network of terminals and synapses containing GABA, GABAAα1 and GABAB1 receptors.
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About the authors
L. I. Khozhai
I. P.Pavlov RAS Institute of Physiology
Email: astarta0505@mail.ru
N. V. Ilyichova
RAS Institute of Cytology
Email: nad9009@yandex.ru
References
- Хожай Л. И., Отеллин В. А. Участие серотонина в механизмах становления двигательного ядра тройничного нерва // Морфология. 2012. Т. 142, вып. 5. С. 23-26.
- Хожай Л. И., Шишко Т. Т. Изменение структурной организации бледного ядра шва при снижении содержания эндогенного серотонина в пренатальный период развития у крыс // Морфология. 2013. Т. 143, вып. 2. С. 75-78.
- Хожай Л. И., Шишко Т. Т., Отеллин В. А. Недостаточность серотонинергической системы в пренатальный период вызывает нарушение становления nucleus retroambiguus у крыс // Журн. эволюц. биохим. 2014. Т. 50, № 2. С. 162-165.
- Alheid G. F., McCrimmon D. R. The chemical neuroanatomy of breathing // Respir. Physiol. Neurobiol. 2008. Vol. 16. P. 3-11.
- Duffin J., Alphen J. Cross-correlation of augmenting expiratory neurons Bötzinger complex in the cat // Exp. Brain Res. 1995. Vol. 103, № 2. P. 251-255.
- Duffin J., Tian G. F., Peever J. H. Functional synaptic connections among respiratory neurons // Respir. Physiol. 2000. Vol. 122, № 2-3. P. 237-246.
- Ezure K., Tanaka J., Saito Y. Activity of brainstem respiratory neurone just before the expiration-inspiration transition in the rat // J. Physiol. 2003. Vol. 547, № 2. P. 629-640.
- Ezure K., Tanaka J., Saito Y. Brainstem and spinal projection of augmenting expiratary neurons in the rat // Neurosci. Res. 2003. Vol. 45, № 1. P. 41-51.
- Fedorco L., Merrill E. G., Lipski J. Two descending medullary inspiratory partways in phrenic motoneurones // Neurosci. Left. 1984. Vol. 43. P. 74-77.
- Guthmann A., Fritschy J. M., Ottersen O. P. et al. GABA, GABA transporters, GABA (A) receptor subunits and GAD mRNAs in the rat parabrachial and Kölliker-Fuse nuclei // J. Comp. Neurol. 1998. Vol. 400, № 2. P. 229-243.
- Heck W. L., Basaraba A. M., Slusarczyk A., Schweitzer L. Early GABA-A receptor clustering during the development of the rostral nucleus of the solitary tract // J. Anat. 2003. Vol. 202, № 4. P. 387-396.
- Kuwana S., Okada Y., Sugawara Y. et al. Disturbance of neural respiratory control in neonatal mice lacking GABA synthesizing enzyme 67-kDa isoform of glutamic acid decarboxylase // Neuroscience. 2003. Vol. 120, № 3. P. 861-870.
- Merrill E. G., Fedorko L. Monosynaptic inhibition of phrenic motoneurons: a long descending projection from Bötzinger neurons // J. Neurosci. 1984. Vol. 4, № 9. P. 2350-2353.
- Paxinos G., Watson C. The rat brain in stereotaxic coordinates. 5nd edn. Sydney: Academic Press, 2004.
- Portillo F., Nunez-Abades P.A. Distribution of bulbospinal neurons supplying bilateral innervation to the phrenic nucleus in the rat // Brain Res. 1992. Vol. 583, № 1-2. P. 349-355.
- Ramirez J. M. Respiratory rhythm generation in mammals: synaptic and membrane properties // Respir. Physiol. 1997. Vol. 110. P. 71-85.
- Ritter B., Zhang W. Early postnatal maturation of GABAA-mediated inhibition in the brainstem respiratory rhythm-generating network of the mouse // Eur. J. Neurosci. 2000. Vol. 12. P. 2975-2984.
- Song G., Li Q., Shao F. Z. GABAergic neurons in Kölliker-Fuse nucleus and Bötzinger complex with axons projecting to phrenic nucleus // Sheng Li Xue Bao. 2000. Vol. 52, № 2. P. 167-169.
- Thomas P., Mortensen M., Hosie A. M., Smart T. G. Dynamic mobility of functional GABA-A receptors at inhibitory synapses // Nat. Neurosci. 2005. Vol. 8, № 7. P. 889-897.
- Wong-Riley M. T., Liu Q. Neurochemical development of brain stem nuclei involved in the control of respiration // Respir. Physiol. Neurobiol. 2005. Vol. 149, № 1-3. P. 83-98.
- Yu S. Y., Wang G. M., Wang H. et al. Raphe pallidus modulates Botzinger complex-induced inhibition of the phrenic nerve in rats // Eur. J. Neurosci. 2011. Vol. 34, № 7. P. 1113-1120.
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