DISTRIBUTION OF NEUROFILAMENT LIGHT CHAIN PROTEINS IN RAT BRAIN CINGULATE CORTEX



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective: immunohistochemical study of the distribution of neurofilament (NF) light chain proteins in the cingulate cortex and adjacent areas of rat forebrain. Material and methods. Distribution of NF light chain proteins in rat cingulate cortex and adjacent areas of the forebrain was studied immunohistochemically on adult male Wistar rats (7 animals) using monoclonal antibodies specific for 70 kDa NF (2F11 clone). Results. NF light chain proteins were found to have irregular distribution in the brain with the maximal expression in the singular neural pathways and the white matter of motor, somatosensory, and insular cortex. Intense 2F11-immunoreactivity was observed in the dorsal (but not ventral) part of the corpus callosum, thus, indicating the difference between the axons of the ventral part of the corpus callosum and nerve fibers of other pathways in their content of NF light chain proteins. Among the brain grey matter areas, most pronounced 2F11-immunoreactivity was found in the cingulate and retrosplenial cortex, where a distinct laminar pattern of immunoreactivity was observed with two layers of intensive expression of NF proteins alternating with much weaker stained layers. Laminar pattern of NF distribution was not found in the neighboring secondary motor cortex, which was immunostained as intensively as the cingulate and retrosplenial ones, but homogeneously. More lateral neocortical areas exhibited very low NF immunoreactivity. Conclusions. The present study demonstrated a laminar pattern of NF-containing nerve fiber distribution in the cingulate and retrosplenial cortex as well as their irregular distribution in the corpus callosum and the effectiveness of antibodies to light chain proteins of neurofilaments (2F11 clone) for labeling the nerve fibers in neuroanatomical studies and analysis of brain myeloarchitectonics.

Full Text

Restricted Access

About the authors

I. P. Grigoriyev

Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology 12 Academician Pavlov Str., St. Petersburg 197376

O. S. Alekseyeva

Institute of Experimental Medicine; I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology; Laboratory of Comparative Respiratory Physiology 12 Academician Pavlov Str., St. Petersburg 197376; 44 Moris Torez Avenue, St. Petersburg 194223

O. V. Kirik

Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology 12 Academician Pavlov Str., St. Petersburg 197376

D. A. Sufiyeva

Institute of Experimental Medicine

Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology 12 Academician Pavlov Str., St. Petersburg 197376

D. E. Korzhevskiy

Institute of Experimental Medicine

Email: iemmorphol@yandex.ru
Laboratory of Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology 12 Academician Pavlov Str., St. Petersburg 197376

References

  1. Григорьев И. П., Шкляева М. А., Кирик О. В., Гилерович Е. Г., Коржевский Д. Э. Распределение альфа-тубулина в структурах переднего мозга крысы // Морфология. 2013. Т. 143, вып. 1. С. 7-10. doi: 10.1007/s11055-013-9864-3.
  2. Козловский С. А., Величковский Б. Б., Вартанов А. В., Никонова Е. Ю., Величковский Б. М. Роль областей цингулярной коры в функционировании памяти человека // Экспериментальная психология. 2012. Т. 5, № 1. С. 12-22.
  3. Коржевский Д. Э., Кирик О. В., Петрова Е. С., Карпенко М. Н., Григорьев И. П., Сухорукова Е. Г., Колос Е. А., Гиляров А. В. Теоретические основы и практическое применение методов иммуногистохимии (2-е издание, исправленное и дополненное) / Под ред. Д. Э. Коржевского. СПб., 2014.
  4. Коржевский Д. Э., Сухорукова Е. Г., Гилерович Е. Г., Петрова Е. С., Кирик О. В., Григорьев И. П. Преимущества и недостатки цинк-этанол-формальдегида как фиксатора для иммуноцитохимических исследований и конфокальной лазерной микроскопии // Морфология. 2013. Т. 143, вып. 2. С. 81-85. doi: 10.1007/s11055-014-9948-8.
  5. Логвинов А. К., Кириченко Е. Ю., Повилайтите П. Е., Сухов А. Г. Структурная организация баррельной коры мозга крысы (иммуногистохимическое исследование) // Морфология. 2010. Т. 137, вып. 1. С. 10-13.
  6. Петрова Е. С., Павлова Н. В., Коржевский Д. Э. Современные морфологические подходы к изучению регенерации периферических нервных проводников // Мед. Акад. Журн. 2012. Т. 12, № 3. С. 15-29.
  7. Федосихина Л. А. Ультраструктурная характеристика первого слоя коры поясной области большого мозга крысы // Арх. анат. 1984. Т. 86, вып. 2. С. 22-28. doi: 10.1007/ BF01148178.
  8. Brodmann K. Vergleichende Lokalisationslehre der Grosshirnrinde: in ihren Prinzipien dargestellt auf Grund des Zellenbaues. Leipzig: Johann Ambrosius Barth Verlag, 1909.
  9. Devinsky O., Morrell M. J., Vogt B. A. Contributions of anterior cingulate cortex to behavior // Brain. 1995. Vol. 118, № 1. P. 279-306. doi: 10.1093/brain/118.1.279.
  10. Etkin A., Egner T., Kalisch R. Emotional processing in anterior cingulate and medial prefrontal cortex // Trends Cogn. Sci. 2011. Vol. 15, № 2. P. 85-93. doi: 10.1016/j.tics.2010.11.004.
  11. Fressinaud C., Jean I., Dubas F. Lesion mechanism dependent, differential changes in neurofilaments and microtubules: a pathological and experimental study // Rev. Neurol. (Paris). 2005. Vol. 161, № 1. P. 55-60. Dоi: RN-01-2005-161-1-0035-3787101019-ART06.
  12. Gray E., Rice C., Nightingale H., Ginty M., Hares K., Kemp K., Cohen N., Love S., Scolding N., Wilkins A. Accumulation of cortical hyperphosphorylated neurofilaments as a marker of neurodege neration in multiple sclerosis // Mult. Scler. 2013. Vol. 19, № 2. P. 153-161. doi: 10.1177/1352458512451661.
  13. Hamani C., Mayberg H., Stone S., Laxton A., Haber S., Loza no A. M. The subcallosal cingulate gyrus in the context of major depression // Biol. Psychiatry. 2011. Vol. 69, № 4. P. 301-308. doi: 10.1016/j.biopsych.2010.09.034
  14. Kobek M., Skowronek R., Jankowski Z., Pałasz A. Neuro filaments and traumatic brain injury // Arch. Med. Sadowej. Kryminol. 2014. Vol. 64, № 4. P. 268-279. doi: 10.5114/amsik.2014.50531.
  15. Lopez-Picon F. R., Uusi-Oukari M., Holopainen I. E. Differential expression and localization of the phosphorylated and nonphosphorylated neurofilaments during the early postnatal development of rat hippocampus // Hippocampus. 2003. Vol. 13, № 7. P. 767-779. doi: 10.1002/hipo.10122.
  16. Paxinos G., Watson C. The rat brain in stereotaxic coordinates. 6th edition. San Diego: Acad. Press, 2007. 451 р.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies