MORPHOMETRIC ANALYSIS OF THE ULTRASTRUCTURE OF HEPATOCYTE MITOCHONDRIA IN THE INTAСT RATS



Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective - сomplex morphometric evaluation of the ultrastructure of mitochondria of hepatocytes in liver sections of the intact rats. Materials and methods. The study was carried out on 5 albino outbred male rats weighing 232.5±20.35 g. Morphometric evaluation of the ultrastructure of mitochondria of hepatocytes on sections of the liver of intact rats performed with the electron microscope JEM-1011 and the iTEM software package (Ver. 5.0). Results. Mitochondria in hepatocytes of sexually mature non-linear intact rats are numerous and polymorphous, have a branched form and in sections occupy about 1/3 of the area of their cytoplasm. High content of elongated mitochondria was noted, which, together with the numerous cristae and a matrix of moderate electron density, are considered to be the signs of high functional activity. The results of the mitochondrial measurements of mitochondria in hepatocytes of intact rats performed with the iTEM program are presented. Conclusions. The morphometric characterization of hepatocyte mitochondria of the intact outbred rats can be used for comparative evaluation of the changes in the mitochondrial structure that arise in the modeling of various pathological conditions, including drug-induced liver disease.

Full Text

Restricted Access

About the authors

M. N. Kurbat

Grodno State Medical University

Email: vwmisha@mail.ru
Scientific Research Laboratory 80 Gorkiy St., Grodno, Belarus 230009

R. I. Kravchuk

Grodno State Medical University

Scientific Research Laboratory 80 Gorkiy St., Grodno, Belarus 230009

O. B. Ostrovskaya

Grodno State Medical University

Scientific Research Laboratory 80 Gorkiy St., Grodno, Belarus 230009

References

  1. Безбородкина Н. Н., Оковитый С. В., Кудрявцева М. В., Кирик О. В., Зарубина И. В., Кудрявцев Б. И. Морфометрия митохондриального аппарата гепатоцитов нормальной и цирротически измененной печени крыс // Цитология. 2008. Т. 50, № 3. С. 228-235.
  2. Babbar M., Sheikh M. S. Metabolic stress and disorders related to alterations in mitochondrial fission or fusion // Mol. Cell Pharmacol. 2013. Vol. 3, № 5. P. 109-133.
  3. Berman S. B., Pineda F. J., Hardwick J. M. Mitochondrial fission and fusion dynamics: the long and short of it // Cell Death Differ. 2008. Vol. 7, № 15. Р. 1147-1152. doi: 10.1038/cdd.2008.57.
  4. Cagalinec M., Safiulina D., Liiv M., Liiv J., Choubey V., Wareski P., Veksler V., Kaasik A. Principles of the mitochondrial fusion and fission cycle in neurons // J. Cell Science. 2013. Vol. 126. Р. 2187-2197. doi: 10.1242/jcs.118844.
  5. Dragovic S., Vermeulen N. P. E., Gerets H. H., Hewitt P. G., Ingel man-Sundberg M., Park B. K., Juhila S., Snoeys J., Weaver R. J. Evidence-based selection of training compounds for use in the mechanism-based integrated prediction of drug-induced liver injury in man // Arch. Toxicol. 2016. Vol. 90. Р. 2979-3003. doi: 10.1007/s00204-016-1845-1.
  6. Finsterer J., Zarrouk-Mahjoub S. Mitochondrial toxicity of cardiac drugs and its relevance to mitochondrial disorders // Expert Opin. Drug Metab. Toxicol. 2015. Vol. 1, № 11. Р. 15-24. doi: 10.1517/17425255.2015.973401.
  7. Friedman J. R., Nunnari J. Mitochondrial form and function // Nature. 2014. Vol. 505, № 7483. P. 335-343. doi: 10.1038/ nature12985.
  8. Fujioka H., Tandler B., Consolo M. C., Karnik P. Division of mitochondria in cultured human fibroblasts // Microsc. Res. Tech. 2013. Vol. 12, № 76. P. 1213-1216. doi: 10.1002/jemt.22287.
  9. Fujioka H., Tandler B., Hoppel C. L. Mitochondrial Division in Rat Cardiomyocytes: An Electron Microscope Study // Anat. Rec. (Hoboken). 2012. Vol. 9, № 295. P. 1455-1461. doi: 10.1002/ ar.22523.
  10. Galloway Ch.A., Lee H., Yoon Y. Mitochondrial morphology - Emerging role in bioenergetics // Free Radic. Biol. Med. 2012. Vol. 12, № 53. P. 2218-2228. doi: 10.1016/j.freeradbiomed.2012.09.035.
  11. Hu D., Wu C. Q., Li Z. J., Liu Y., Fan X., Wang Q. J., Ding R. G. Characterizing the mechanism of thiazolidinedione-induced hepatotoxicity: An in vitro model in mitochondria // Toxicol Appl. Pharmacol. 2015. Vol. 2, № 284. P. 134-141. doi: 10.1016/j. taap.2015.02.018.
  12. Kroemer G., Galluzz L., Brenner C. Mitochondrial membrane permeabilization in cell death // Physiological Reviews. 2007. Vol. 87, № 1. Р. 99-163.
  13. Malhi H., Guicciardi M. E., Gores G. J. Hepatocyte death: a clear and present danger // Physiological Reviews. 2010. Vol. 90, № 3. Р. 1165-1194. doi: 10.1152/physrev.00061.2009.
  14. Mannella C. A. The relevance of mitochondrial membrane topology to mitochondrial function // Biochimica et Biophysica Acta. 2006. № 1762. Р. 140-147. doi: 10.1016/j.bbadis.2005.07.001.
  15. Orhan H. Extrahepatic targets and cellular reactivity of drug metabolites // Curr. Med. Chem. 2015. Vol. 4, № 22. Р. 408-437.
  16. Otera H., Ishihara N., Mihara K. New insights into the function and regulation of mitochondrial fission // Biochimica et Biophysica Acta. 2013. № 1833. Р. 1256-1268. doi: 10.1016/j. bbamcr.2013.02.002.
  17. Otera H., Mihara K. Molecular mechanisms and physiologic functions of mitochondrial dynamics // J. Biochem. 2011. № 149. Р. 241-251. doi: 10.1093/jb/mvr002.
  18. Picard M., White K., Turnbull D. M. Mitochondrial morphology, topology, and membrane interactions in skeletal muscle: a quantitative three-dimensional electron microscopy study // J. Appl. Physiol. 2013. № 114. Р. 161-171. doi: 10.1152/japplphysiol.01096.2012.
  19. Shaughnessy D. T., McAllister K., Worth L., Haugen A. C., Meyer J. N., Domann F. E., Van Houten B., Mostoslavsky R., Bult man S. J., Baccarelli A. A., Begley T. J., Sobol R. W., Hir schey M. D., Ideker T., Santos J. H., Copeland W. C., Tice R. R., Balshaw D. M., Tyson F. L. Mitochondria, Energetics, Epi genetics, and Cellular Responses to Stress // Environmental Health Perspectives. 2014. Vol. 122, № 12. Р. 1271-1278. doi: 10.1289/ehp.1408418.
  20. Westermann B. Mitochondrial fusion and fission in cell life and death // Nat. Rev. Mol. Cell Biol. 2010. № 11. Р. 872-884. doi: 10.1038/nrm3013.
  21. Yamaguchi R., Perkins G. Dynamics of mitochondrial structure during apoptosis and the enigma of Opa1 // Biochim. Biophys. Acta. 2009. Vol. 8, № 1787. P. 963-972. doi: 10.1016/j. bbabio.2009.02.005.
  22. Zick M., Rabl R., Reichert A. S. Cristae formation-linking ultrastructure and function of mitochondria // Biochimica et Biophysica Acta. 2009. № 1793. P. 5-19. doi: 10.1016/j.bbamcr.2008.06.013.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Eco-Vector


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: № 0110212 от 08.02.1993.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies